Exercise-induced dehydration with and without environmental heat stress results in increased oxidative stress

2.50
Hdl Handle:
http://hdl.handle.net/10547/295122
Title:
Exercise-induced dehydration with and without environmental heat stress results in increased oxidative stress
Authors:
Hillman, Angela R.; Vince, Rebecca V.; Taylor, Lee; McNaughton, Lars; Mitchell, Nigel; Siegler, Jason C.
Abstract:
While in vitro work has revealed that dehydration and hyperthermia can elicit increased cellular and oxidative stress, in vivo research linking dehydration, hyperthermia, and oxidative stress is limited. The purpose of this study was to investigate the effects of exercise-induced dehydration with and without hyperthermia on oxidative stress. Seven healthy male, trained cyclists (power output (W) at lactate threshold (LT): 199 ± 19 W) completed 90 min of cycling exercise at 95% LT followed by a 5-km time trial (TT) in 4 trials: (i) euhydration in a warm environment (EU-W, control), (ii) dehydration in a warm environment (DE-W), (iii) euhydration in a thermoneutral environment (EU-T), and (iv) dehydration in a thermoneutral environment (DE-T) (W: 33.9 ± 0.9 °C; T: 23.0 ± 1.0 °C). Oxidized glutathione (GSSG) increased significantly postexercise in dehydration trials only (DE-W: p < 0.01, DE-T: p = 0.03), and while not significant, total glutathione (TGSH) and thiobarbituric acid reactive substances (TBARS) tended to increase postexercise in dehydration trials (p = 0.08 for both). Monocyte heat shock protein 72 (HSP72) concentration was increased (p = 0.01) while lymphocyte HSP32 concentration was decreased for all trials (p = 0.02). Exercise-induced dehydration led to an increase in GSSG concentration while maintenance of euhydration attenuated these increases regardless of environmental condition. Additionally, we found evidence of increased cellular stress (measured via HSP) during all trials independent of hydration status and environment. Finally, both 90-min and 5-km TT performances were reduced during only the DE-W trial, likely a result of combined cellular stress, hyperthermia, and dehydration. These findings highlight the importance of fluid consumption during exercise to attenuate thermal and oxidative stress during prolonged exercise in the heat.
Citation:
Hillman, A.R., Vince, R., Taylor, L., McNaughton, L., Mitchell, N. and Sigler, J. (2011) 'Exercise-induced dehydration with and without environmental heat stress results in increased oxidative stress', Applied Physiology, Nutrition, and Metabolism, 36(5), pp.698-706
Publisher:
NRC Research Press
Journal:
Applied Physiology, Nutrition, and Metabolism
Issue Date:
2011
URI:
http://hdl.handle.net/10547/295122
DOI:
10.1139/h11-080
Additional Links:
http://www.nrcresearchpress.com/doi/abs/10.1139/h11-080
Type:
Article
Language:
en
ISSN:
1715-5312; 1715-5320
Appears in Collections:
Applied Sport and Exercise Physiology

Full metadata record

DC FieldValue Language
dc.contributor.authorHillman, Angela R.en_GB
dc.contributor.authorVince, Rebecca V.en_GB
dc.contributor.authorTaylor, Leeen_GB
dc.contributor.authorMcNaughton, Larsen_GB
dc.contributor.authorMitchell, Nigelen_GB
dc.contributor.authorSiegler, Jason C.en_GB
dc.date.accessioned2013-07-03T08:04:58Z-
dc.date.available2013-07-03T08:04:58Z-
dc.date.issued2011-
dc.identifier.citationHillman, A.R., Vince, R., Taylor, L., McNaughton, L., Mitchell, N. and Sigler, J. (2011) 'Exercise-induced dehydration with and without environmental heat stress results in increased oxidative stress', Applied Physiology, Nutrition, and Metabolism, 36(5), pp.698-706en_GB
dc.identifier.issn1715-5312-
dc.identifier.issn1715-5320-
dc.identifier.doi10.1139/h11-080-
dc.identifier.urihttp://hdl.handle.net/10547/295122-
dc.description.abstractWhile in vitro work has revealed that dehydration and hyperthermia can elicit increased cellular and oxidative stress, in vivo research linking dehydration, hyperthermia, and oxidative stress is limited. The purpose of this study was to investigate the effects of exercise-induced dehydration with and without hyperthermia on oxidative stress. Seven healthy male, trained cyclists (power output (W) at lactate threshold (LT): 199 ± 19 W) completed 90 min of cycling exercise at 95% LT followed by a 5-km time trial (TT) in 4 trials: (i) euhydration in a warm environment (EU-W, control), (ii) dehydration in a warm environment (DE-W), (iii) euhydration in a thermoneutral environment (EU-T), and (iv) dehydration in a thermoneutral environment (DE-T) (W: 33.9 ± 0.9 °C; T: 23.0 ± 1.0 °C). Oxidized glutathione (GSSG) increased significantly postexercise in dehydration trials only (DE-W: p < 0.01, DE-T: p = 0.03), and while not significant, total glutathione (TGSH) and thiobarbituric acid reactive substances (TBARS) tended to increase postexercise in dehydration trials (p = 0.08 for both). Monocyte heat shock protein 72 (HSP72) concentration was increased (p = 0.01) while lymphocyte HSP32 concentration was decreased for all trials (p = 0.02). Exercise-induced dehydration led to an increase in GSSG concentration while maintenance of euhydration attenuated these increases regardless of environmental condition. Additionally, we found evidence of increased cellular stress (measured via HSP) during all trials independent of hydration status and environment. Finally, both 90-min and 5-km TT performances were reduced during only the DE-W trial, likely a result of combined cellular stress, hyperthermia, and dehydration. These findings highlight the importance of fluid consumption during exercise to attenuate thermal and oxidative stress during prolonged exercise in the heat.en_GB
dc.language.isoenen
dc.publisherNRC Research Pressen_GB
dc.relation.urlhttp://www.nrcresearchpress.com/doi/abs/10.1139/h11-080en_GB
dc.rightsArchived with thanks to Applied Physiology, Nutrition, and Metabolismen_GB
dc.subjectC600 Sports Scienceen_GB
dc.titleExercise-induced dehydration with and without environmental heat stress results in increased oxidative stressen
dc.typeArticleen
dc.identifier.journalApplied Physiology, Nutrition, and Metabolismen_GB
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