Microparticle-associated vascular adhesion molecule-1 and tissue factor follow a circadian rhythm in healthy human subjects

2.50
Hdl Handle:
http://hdl.handle.net/10547/295058
Title:
Microparticle-associated vascular adhesion molecule-1 and tissue factor follow a circadian rhythm in healthy human subjects
Authors:
Madden, Leigh A.; Vince, Rebecca V.; Sandström, Marie E.; Taylor, Lee; McNaughton, Lars; Laden, Gerard
Abstract:
An increased risk of death or severe injury due to late-morning thrombotic events is well established. Tissue factor (TF) is the initiator of the coagulation cascade, and endothelial stresses, coupled with production of pro-coagulant microparticles (MP) are also important factors in loss of haemostasis. TF and vascular cell adhesion molecule-1 (VCAM-1) -positive cell microparticles were assessed periodically over a 24-hour (h) period in healthy human subjects to ascertain if they followed a circadian rhythm. Eleven healthy male subjects were assessed in a temperature-controlled environment with dietary intake consistent between subjects. Blood samples were taken every 4 h by venipuncture, and TF and VCAM-1 positive microparticles were quantified by flow cytometry. A significant circadian rhythm was observed in VCAM-1 MP (p=or<0.0001), and a trend was shown, although not statistically significant (p=0.065) in TF microparticles. A peak was observed at 9 a.m. for VCAM-1 positive MP, followed by a decrease and subsequent peak at 9 p.m. and a minimum at 5 a.m. TF-positive MP followed a strikingly similar trend in both variation and absolute numbers with a delay. A circadian rhythm was observed in VCAM-1 and less so TF-positive MP. This has significant implications in terms of the well known increased risk of cardiovascular thrombotic events matching this data. To our knowledge this is the first such report of quantified measurements of these MP over a 24-h period and the only measurement of a 24-h variation of in-vivo blood-borne TF.
Citation:
Madden, L. A., Vince, R. V., Sandstrom, M. E., Taylor, L., McNaughton, L. and Laden, G. (2008) 'Microparticle-associated vascular adhesion molecule-i and tissue factor follow a circadian rhythm in healthy human subjects', Thrombosis and Haemostasis, 99, pp. 909-915.
Publisher:
Schattauer
Journal:
Thrombosis and haemostasis
Issue Date:
May-2008
URI:
http://hdl.handle.net/10547/295058
DOI:
10.1160TH008-01-0030
PubMed ID:
18449421
Additional Links:
http://www.ncbi.nlm.nih.gov/pubmed/18449421
Type:
Article
Language:
en
ISSN:
0340-6245
Appears in Collections:
Applied Sport and Exercise Physiology

Full metadata record

DC FieldValue Language
dc.contributor.authorMadden, Leigh A.en_GB
dc.contributor.authorVince, Rebecca V.en_GB
dc.contributor.authorSandström, Marie E.en_GB
dc.contributor.authorTaylor, Leeen_GB
dc.contributor.authorMcNaughton, Larsen_GB
dc.contributor.authorLaden, Gerarden_GB
dc.date.accessioned2013-07-02T15:57:19Z-
dc.date.available2013-07-02T15:57:19Z-
dc.date.issued2008-05-
dc.identifier.citationMadden, L. A., Vince, R. V., Sandstrom, M. E., Taylor, L., McNaughton, L. and Laden, G. (2008) 'Microparticle-associated vascular adhesion molecule-i and tissue factor follow a circadian rhythm in healthy human subjects', Thrombosis and Haemostasis, 99, pp. 909-915.en_GB
dc.identifier.issn0340-6245-
dc.identifier.pmid18449421-
dc.identifier.doi10.1160TH008-01-0030-
dc.identifier.urihttp://hdl.handle.net/10547/295058-
dc.description.abstractAn increased risk of death or severe injury due to late-morning thrombotic events is well established. Tissue factor (TF) is the initiator of the coagulation cascade, and endothelial stresses, coupled with production of pro-coagulant microparticles (MP) are also important factors in loss of haemostasis. TF and vascular cell adhesion molecule-1 (VCAM-1) -positive cell microparticles were assessed periodically over a 24-hour (h) period in healthy human subjects to ascertain if they followed a circadian rhythm. Eleven healthy male subjects were assessed in a temperature-controlled environment with dietary intake consistent between subjects. Blood samples were taken every 4 h by venipuncture, and TF and VCAM-1 positive microparticles were quantified by flow cytometry. A significant circadian rhythm was observed in VCAM-1 MP (p=or<0.0001), and a trend was shown, although not statistically significant (p=0.065) in TF microparticles. A peak was observed at 9 a.m. for VCAM-1 positive MP, followed by a decrease and subsequent peak at 9 p.m. and a minimum at 5 a.m. TF-positive MP followed a strikingly similar trend in both variation and absolute numbers with a delay. A circadian rhythm was observed in VCAM-1 and less so TF-positive MP. This has significant implications in terms of the well known increased risk of cardiovascular thrombotic events matching this data. To our knowledge this is the first such report of quantified measurements of these MP over a 24-h period and the only measurement of a 24-h variation of in-vivo blood-borne TF.en_GB
dc.language.isoenen
dc.publisherSchattaueren_GB
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pubmed/18449421en_GB
dc.rightsArchived with thanks to Thrombosis and haemostasisen_GB
dc.subjectC600 Sports Scienceen_GB
dc.subject.meshAdolescent-
dc.subject.meshAdult-
dc.subject.meshBiological Markers-
dc.subject.meshCardiovascular Diseases-
dc.subject.meshCell Membrane Structures-
dc.subject.meshCircadian Rhythm-
dc.subject.meshEnzyme-Linked Immunosorbent Assay-
dc.subject.meshFlow Cytometry-
dc.subject.meshHumans-
dc.subject.meshMale-
dc.subject.meshReference Values-
dc.subject.meshThromboplastin-
dc.subject.meshVascular Cell Adhesion Molecule-1-
dc.titleMicroparticle-associated vascular adhesion molecule-1 and tissue factor follow a circadian rhythm in healthy human subjectsen
dc.typeArticleen
dc.identifier.journalThrombosis and haemostasisen_GB

Related articles on PubMed

All Items in UOBREP are protected by copyright, with all rights reserved, unless otherwise indicated.