Hypoxia mediated release of endothelial microparticles and increased association of S100A12 with circulating neutrophils

2.50
Hdl Handle:
http://hdl.handle.net/10547/294856
Title:
Hypoxia mediated release of endothelial microparticles and increased association of S100A12 with circulating neutrophils
Authors:
Vince, Rebecca V.; Chrismas, Bryna; Midgley, Adrian W.; McNaughton, Lars R.; Madden, Leigh A.
Abstract:
Microparticles are released from the endothelium under normal homeostatic conditions and have been shown elevated in disease states, most notably those characterised by endothelial dysfunction. The endothelium is sensitive to oxidative stress/status and vascular cell adhesion molecule-1 (VCAM-1) expression is upregulated upon activated endothelium, furthermore the presence of VCAM-1 on microparticles is known. S100A12, a calcium binding protein part of the S100 family, is shown to be present on circulating leukocytes and is thought a sensitive marker to local inflammatory process, which may be driven by oxidative stress. Eight healthy males were subjected to breathing hypoxic air (15% O(2), approximately equivalent to 3000 metres altitude) for 80 minutes in a temperature controlled laboratory and venous blood samples were processed immediately for VCAM-1 microparticles (VCAM-1 MP) and S100A12 association with leukocytes by flow cytometry. A pre-hypoxic blood sample was used for comparison. Both VCAM-1 MP and S100A12 association with neutrophils were significantly elevated post hypoxic breathing later declining to levels observed in the pre-test samples. A similar trend was observed in both cases and a correlation may exist between these two markers in response to hypoxia. These data offer evidence using novel markers of endothelial and circulating blood responses to hypoxia.
Citation:
Vince, R.V., Chrismas, B.C., Midgley, A.W., McNaughton, L.R., & Madden, L.A. (2009) 'Hypoxia mediated release of endothelial microparticles and increased association of S100A12 with circulating neutrophils', Oxidative Medicine and Cellular Longevity, 2(1), pp. 2-6
Publisher:
Hindawi Publishing Corporation
Journal:
Oxidative medicine and cellular longevity
Issue Date:
2009
URI:
http://hdl.handle.net/10547/294856
PubMed ID:
20046638
Additional Links:
http://www.ncbi.nlm.nih.gov/pubmed/20046638
Type:
Article
Language:
en
ISSN:
1942-0994
Appears in Collections:
Applied Sport and Exercise Physiology

Full metadata record

DC FieldValue Language
dc.contributor.authorVince, Rebecca V.en_GB
dc.contributor.authorChrismas, Brynaen_GB
dc.contributor.authorMidgley, Adrian W.en_GB
dc.contributor.authorMcNaughton, Lars R.en_GB
dc.contributor.authorMadden, Leigh A.en_GB
dc.date.accessioned2013-06-28T11:09:41Z-
dc.date.available2013-06-28T11:09:41Z-
dc.date.issued2009-
dc.identifier.citationVince, R.V., Chrismas, B.C., Midgley, A.W., McNaughton, L.R., & Madden, L.A. (2009) 'Hypoxia mediated release of endothelial microparticles and increased association of S100A12 with circulating neutrophils', Oxidative Medicine and Cellular Longevity, 2(1), pp. 2-6en_GB
dc.identifier.issn1942-0994-
dc.identifier.pmid20046638-
dc.identifier.urihttp://hdl.handle.net/10547/294856-
dc.description.abstractMicroparticles are released from the endothelium under normal homeostatic conditions and have been shown elevated in disease states, most notably those characterised by endothelial dysfunction. The endothelium is sensitive to oxidative stress/status and vascular cell adhesion molecule-1 (VCAM-1) expression is upregulated upon activated endothelium, furthermore the presence of VCAM-1 on microparticles is known. S100A12, a calcium binding protein part of the S100 family, is shown to be present on circulating leukocytes and is thought a sensitive marker to local inflammatory process, which may be driven by oxidative stress. Eight healthy males were subjected to breathing hypoxic air (15% O(2), approximately equivalent to 3000 metres altitude) for 80 minutes in a temperature controlled laboratory and venous blood samples were processed immediately for VCAM-1 microparticles (VCAM-1 MP) and S100A12 association with leukocytes by flow cytometry. A pre-hypoxic blood sample was used for comparison. Both VCAM-1 MP and S100A12 association with neutrophils were significantly elevated post hypoxic breathing later declining to levels observed in the pre-test samples. A similar trend was observed in both cases and a correlation may exist between these two markers in response to hypoxia. These data offer evidence using novel markers of endothelial and circulating blood responses to hypoxia.en_GB
dc.language.isoenen
dc.publisherHindawi Publishing Corporationen_GB
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pubmed/20046638en_GB
dc.rightsArchived with thanks to Oxidative medicine and cellular longevityen_GB
dc.subjectRAGEen_GB
dc.subjectendotheliumen_GB
dc.subjecthypoxiaen_GB
dc.subjectoxidative stressen_GB
dc.subjectC600 Sports Scienceen_GB
dc.subject.meshAdolescent-
dc.subject.meshAnoxia-
dc.subject.meshCell-Derived Microparticles-
dc.subject.meshEndothelium, Vascular-
dc.subject.meshFlow Cytometry-
dc.subject.meshHumans-
dc.subject.meshMale-
dc.subject.meshNeutrophils-
dc.subject.meshS100 Proteins-
dc.subject.meshVascular Cell Adhesion Molecule-1-
dc.subject.meshYoung Adult-
dc.titleHypoxia mediated release of endothelial microparticles and increased association of S100A12 with circulating neutrophilsen
dc.typeArticleen
dc.identifier.journalOxidative medicine and cellular longevityen_GB

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