2.50
Hdl Handle:
http://hdl.handle.net/10547/294571
Title:
A role for glycine in the gating of plant NMDA-like receptors
Authors:
Dubos, Christian; Huggins, David; Grant, Guy H.; Knight, Marc R.; Campbell, Malcolm M.
Abstract:
The amino acid glycine has a well-established role in signalling in the mammalian central nervous system. For example, glycine acts synergistically with the major excitatory neurotransmitter, glutamate, to regulate the influx of ions such as calcium, through N-methyl-d-aspartate (NMDA) receptors. Plants possess NMDA-like receptors, generically referred to as glutamate receptors (GLRs), named on the basis of their presumed ligand, glutamate. Previously, glycine has not been implicated in plant GLR activity or any other aspect of plant signalling. Using transgenic Arabidopsis seedlings expressing aequorin to monitor ligand-mediated changes in the cytosolic concentration of Ca2+ ([Ca2+]cyt), the data presented herein show that glutamate and glycine act synergistically to control ligand-mediated gating of calcium in plants. Glutamate and glycine synergism also regulates hypocotyl elongation. Transient increases in [Ca2+]cyt mediated by glutamate and glycine, as well as hypocotyl elongation, were inhibited by 6,7-dinitroquinoxaline-2,3 dione (DNQX), a competitive inhibitor of animal GLRs. Using a multiscale docking algorithm in combination with a molecular model of the ligand-binding domain of plant GLRs, evidence is provided indicating that glycine, and not glutamate, is likely to be the natural ligand for most plant GLR subunits. These findings uncover a hitherto unconsidered role for glycine signalling in plants, and suggest that the synergistic action of glutamate and glycine at NMDA-like receptors predates the divergence of plants and animals.
Citation:
Dubos, C., Huggins, D., Grant, G.H., Knight, M.R. and Campbell, M.M. (2003) 'A role for glycine in the gating of plant NMDA-like receptors', The Plant Journal, 35(6), pp.800-810.
Publisher:
Wiley
Journal:
The Plant Journal
Issue Date:
2003
URI:
http://hdl.handle.net/10547/294571
DOI:
10.1046/j.1365-313X.2003.01849.x
Additional Links:
http://doi.wiley.com/10.1046/j.1365-313X.2003.01849.x
Type:
Article
Language:
en
ISSN:
09607412; 1365313X
Appears in Collections:
Cell and Cryobiology Research Group

Full metadata record

DC FieldValue Language
dc.contributor.authorDubos, Christianen_GB
dc.contributor.authorHuggins, Daviden_GB
dc.contributor.authorGrant, Guy H.en_GB
dc.contributor.authorKnight, Marc R.en_GB
dc.contributor.authorCampbell, Malcolm M.en_GB
dc.date.accessioned2013-06-26T08:26:57Z-
dc.date.available2013-06-26T08:26:57Z-
dc.date.issued2003-
dc.identifier.citationDubos, C., Huggins, D., Grant, G.H., Knight, M.R. and Campbell, M.M. (2003) 'A role for glycine in the gating of plant NMDA-like receptors', The Plant Journal, 35(6), pp.800-810.en_GB
dc.identifier.issn09607412-
dc.identifier.issn1365313X-
dc.identifier.doi10.1046/j.1365-313X.2003.01849.x-
dc.identifier.urihttp://hdl.handle.net/10547/294571-
dc.description.abstractThe amino acid glycine has a well-established role in signalling in the mammalian central nervous system. For example, glycine acts synergistically with the major excitatory neurotransmitter, glutamate, to regulate the influx of ions such as calcium, through N-methyl-d-aspartate (NMDA) receptors. Plants possess NMDA-like receptors, generically referred to as glutamate receptors (GLRs), named on the basis of their presumed ligand, glutamate. Previously, glycine has not been implicated in plant GLR activity or any other aspect of plant signalling. Using transgenic Arabidopsis seedlings expressing aequorin to monitor ligand-mediated changes in the cytosolic concentration of Ca2+ ([Ca2+]cyt), the data presented herein show that glutamate and glycine act synergistically to control ligand-mediated gating of calcium in plants. Glutamate and glycine synergism also regulates hypocotyl elongation. Transient increases in [Ca2+]cyt mediated by glutamate and glycine, as well as hypocotyl elongation, were inhibited by 6,7-dinitroquinoxaline-2,3 dione (DNQX), a competitive inhibitor of animal GLRs. Using a multiscale docking algorithm in combination with a molecular model of the ligand-binding domain of plant GLRs, evidence is provided indicating that glycine, and not glutamate, is likely to be the natural ligand for most plant GLR subunits. These findings uncover a hitherto unconsidered role for glycine signalling in plants, and suggest that the synergistic action of glutamate and glycine at NMDA-like receptors predates the divergence of plants and animals.en_GB
dc.language.isoenen
dc.publisherWileyen_GB
dc.relation.urlhttp://doi.wiley.com/10.1046/j.1365-313X.2003.01849.xen_GB
dc.rightsArchived with thanks to The Plant Journalen_GB
dc.subjectglycineen_GB
dc.subjectionotropic glutamate receptorsen_GB
dc.subjectNMDAen_GB
dc.titleA role for glycine in the gating of plant NMDA-like receptorsen
dc.typeArticleen
dc.identifier.journalThe Plant Journalen_GB
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