2.50
Hdl Handle:
http://hdl.handle.net/10547/293974
Title:
dpp Genes of Rhizobium leguminosarum specify uptake of δ-Aminolevulinic Acid
Authors:
Carter, R. A.; Yeoman, K. H.; Klein, A.; Hosie, Arthur H.F. ( 0000-0002-1327-7901 ) ; Sawers, G.; Poole, Philip S.; Johnston, A. W. B.
Abstract:
An operon with homology to the dppABCDF genes required to transport dipeptides in bacteria was identified in the N2-fixing symbiont, Rhizobium leguminosarum. As in other bacteria, dpp mutants were severely affected in the import of δ-aminolevulinic acid (ALA), a heme precursor. ALA uptake was antagonized by adding dipeptides, indicating that these two classes of molecule share the same transporter. Mutations in dppABCDF did not affect symbiotic N2 fixation on peas, suggesting that the ALA needed for heme synthesis is not supplied by the plant or that another uptake system functions in the bacteroids. The dppABCDF operon of R. leguminosarum resembles that in other bacteria, with a gap between dppA and dppB containing inverted repeats that may stabilize mRNA and may explain why transcription of dppA alone was higher than that of dppBCDF. The dppABCDF promoter was mapped and is most likely recognized by σ70.
Citation:
Carter, R.A., Yeoman, K.H., Klein, A.(2002) 'Dpp genes of Rhizobium Leguminosarum specify uptake of δ-Aminolevulinic Acid', Molecular Plant-Microbe Interactions, 15 (1),pp.69-74
Publisher:
APS PRESS
Journal:
Molecular Plant-Microbe Interactions
Issue Date:
2002
URI:
http://hdl.handle.net/10547/293974
DOI:
10.1094/MPMI.2002.15.1.69
Additional Links:
http://apsjournals.apsnet.org/doi/abs/10.1094/MPMI.2002.15.1.69
Type:
Article
Language:
en
ISSN:
0894-0282
Appears in Collections:
Cell and Cryobiology Research Group

Full metadata record

DC FieldValue Language
dc.contributor.authorCarter, R. A.en_GB
dc.contributor.authorYeoman, K. H.en_GB
dc.contributor.authorKlein, A.en_GB
dc.contributor.authorHosie, Arthur H.F.en_GB
dc.contributor.authorSawers, G.en_GB
dc.contributor.authorPoole, Philip S.en_GB
dc.contributor.authorJohnston, A. W. B.en_GB
dc.date.accessioned2013-06-14T12:31:53Z-
dc.date.available2013-06-14T12:31:53Z-
dc.date.issued2002-
dc.identifier.citationCarter, R.A., Yeoman, K.H., Klein, A.(2002) 'Dpp genes of Rhizobium Leguminosarum specify uptake of δ-Aminolevulinic Acid', Molecular Plant-Microbe Interactions, 15 (1),pp.69-74en_GB
dc.identifier.issn0894-0282-
dc.identifier.doi10.1094/MPMI.2002.15.1.69-
dc.identifier.urihttp://hdl.handle.net/10547/293974-
dc.description.abstractAn operon with homology to the dppABCDF genes required to transport dipeptides in bacteria was identified in the N2-fixing symbiont, Rhizobium leguminosarum. As in other bacteria, dpp mutants were severely affected in the import of δ-aminolevulinic acid (ALA), a heme precursor. ALA uptake was antagonized by adding dipeptides, indicating that these two classes of molecule share the same transporter. Mutations in dppABCDF did not affect symbiotic N2 fixation on peas, suggesting that the ALA needed for heme synthesis is not supplied by the plant or that another uptake system functions in the bacteroids. The dppABCDF operon of R. leguminosarum resembles that in other bacteria, with a gap between dppA and dppB containing inverted repeats that may stabilize mRNA and may explain why transcription of dppA alone was higher than that of dppBCDF. The dppABCDF promoter was mapped and is most likely recognized by σ70.en_GB
dc.language.isoenen
dc.publisherAPS PRESSen_GB
dc.relation.urlhttp://apsjournals.apsnet.org/doi/abs/10.1094/MPMI.2002.15.1.69en_GB
dc.rightsArchived with thanks to Molecular Plant-Microbe Interactionsen_GB
dc.subjectABC transporteren_GB
dc.subjectgluconate kinaseen_GB
dc.titledpp Genes of Rhizobium leguminosarum specify uptake of δ-Aminolevulinic Aciden
dc.typeArticleen
dc.identifier.journalMolecular Plant-Microbe Interactionsen_GB
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