A novel method for the transport and analysis of genetic material from polyps and zooxanthellae of scleractinian corals

2.50
Hdl Handle:
http://hdl.handle.net/10547/293946
Title:
A novel method for the transport and analysis of genetic material from polyps and zooxanthellae of scleractinian corals
Authors:
Crabbe, M. James C.
Abstract:
We have developed a new simple method for transport, storage, and analysis of genetic material from the corals Agaricia agaricites, Dendrogyra cylindrica, Eusmilia ancora, Meandrina meandrites, Montastrea annularis, Porites astreoides, Porites furcata, Porites porites, and Siderastrea siderea at room temperature. All species yielded sufficient DNA from a single FTA® card (19 μg–43 ng) for subsequent PCR amplification of both coral and zooxanthellar DNA. The D1 and D2 variable region of the large subunit rRNA gene (LSUrDNA) was amplified from the DNA of P. furcata and S. siderea by PCR. Electrophoresis yielded two major DNA bands: an 800-base pair (bp) DNA, which represented the coral ribosomal RNA (rRNA) gene, and a 600-bp DNA, which represented the zooxanthellar srRNA gene. Extraction of DNA from the bands yielded between 290 μg total DNA (S. siderea coral DNA) and 9 μg total DNA (P. furcata zooxanthellar DNA). The ability to transport and store genetic material from scleractinian corals without resort to laboratory facilities in the field allows for the molecular study of a far wider range and variety of coral sites than have been studied to date.
Citation:
Crabbe, M.C. (2003) 'A novel method for the transport and analysis of genetic material from polyps and zooxanthellae of scleractinian corals', Journal of Biochemical and Biophysical Methods, 57 (2),pp.171-176
Publisher:
Elsevier
Journal:
Journal of Biochemical and Biophysical Methods
Issue Date:
2003
URI:
http://hdl.handle.net/10547/293946
DOI:
10.1016/S0165-022X(03)00051-4
Additional Links:
http://www.ncbi.nlm.nih.gov/pubmed/12915008
Type:
Article
Language:
en
ISSN:
0165-022X
Appears in Collections:
Environmental Monitoring Research Group

Full metadata record

DC FieldValue Language
dc.contributor.authorCrabbe, M. James C.en_GB
dc.date.accessioned2013-06-13T15:04:51Z-
dc.date.available2013-06-13T15:04:51Z-
dc.date.issued2003-
dc.identifier.citationCrabbe, M.C. (2003) 'A novel method for the transport and analysis of genetic material from polyps and zooxanthellae of scleractinian corals', Journal of Biochemical and Biophysical Methods, 57 (2),pp.171-176en_GB
dc.identifier.issn0165-022X-
dc.identifier.doi10.1016/S0165-022X(03)00051-4-
dc.identifier.urihttp://hdl.handle.net/10547/293946-
dc.description.abstractWe have developed a new simple method for transport, storage, and analysis of genetic material from the corals Agaricia agaricites, Dendrogyra cylindrica, Eusmilia ancora, Meandrina meandrites, Montastrea annularis, Porites astreoides, Porites furcata, Porites porites, and Siderastrea siderea at room temperature. All species yielded sufficient DNA from a single FTA® card (19 μg–43 ng) for subsequent PCR amplification of both coral and zooxanthellar DNA. The D1 and D2 variable region of the large subunit rRNA gene (LSUrDNA) was amplified from the DNA of P. furcata and S. siderea by PCR. Electrophoresis yielded two major DNA bands: an 800-base pair (bp) DNA, which represented the coral ribosomal RNA (rRNA) gene, and a 600-bp DNA, which represented the zooxanthellar srRNA gene. Extraction of DNA from the bands yielded between 290 μg total DNA (S. siderea coral DNA) and 9 μg total DNA (P. furcata zooxanthellar DNA). The ability to transport and store genetic material from scleractinian corals without resort to laboratory facilities in the field allows for the molecular study of a far wider range and variety of coral sites than have been studied to date.en_GB
dc.language.isoenen
dc.publisherElsevieren_GB
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pubmed/12915008en_GB
dc.rightsArchived with thanks to Journal of Biochemical and Biophysical Methodsen_GB
dc.subjectFTA cardsen_GB
dc.subjectDNAen_GB
dc.subjectrRNAen_GB
dc.subjectZooxanthellaeen_GB
dc.subjectCnidariaen_GB
dc.titleA novel method for the transport and analysis of genetic material from polyps and zooxanthellae of scleractinian coralsen
dc.typeArticleen
dc.identifier.journalJournal of Biochemical and Biophysical Methodsen_GB
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