A family of promoter probe vectors incorporating autofluorescent and chromogenic reporter proteins for studying gene expression in gram-negative bacteria

2.50
Hdl Handle:
http://hdl.handle.net/10547/293938
Title:
A family of promoter probe vectors incorporating autofluorescent and chromogenic reporter proteins for studying gene expression in gram-negative bacteria
Authors:
Karunakaran, R.; Mauchline, T.H.; Hosie, Arthur H.F. ( 0000-0002-1327-7901 ) ; Poole, Philip S.
Abstract:
A series of promoter probe vectors for use in Gram-negative bacteria has been made in two broad-host-range vectors, pOT (pBBR replicon) and pJP2 (incP replicon). Reporter fusions can be made to gfpUV, gfpmut3.1, unstable gfpmut3.1 variants (LAA, LVA, AAV and ASV), gfp+, dsRed2, dsRedT.3, dsRedT.4, mRFP1, gusA or lacZ. The two vector families, pOT and pJP2, are compatible with one another and share the same polylinker for facile interchange of promoter regions. Vectors based on pJP2 have the advantage of being ultra-stable in the environment due to the presence of the parABCDE genes. As a confirmation of their usefulness, the dicarboxylic acid transport system promoter (dctAp) was cloned into a pOT (pRU1097)- and a pJP2 (pRU1156)-based vector and shown to be expressed by Rhizobium leguminosarum in infection threads of vetch. This indicates the presence of dicarboxylates at the earliest stages of nodule formation.
Citation:
Karunakaran, R., Mauchline, T. H., Hosie, A.H.F. and Poole, P.S. (2005) 'A family of promoter probe vectors incorporating autofluorescent and chromogenic reporter proteins for studying gene expression in gram-negative bacteria', Microbiology, 151 (10),pp.3249-3256
Publisher:
Society for General Microbiology
Journal:
Microbiology
Issue Date:
2005
URI:
http://hdl.handle.net/10547/293938
DOI:
10.1099/mic.0.28311-0
Additional Links:
http://mic.sgmjournals.org/cgi/doi/10.1099/mic.0.28311-0
Type:
Article
Language:
en
ISSN:
1350-0872; 1465-2080
Appears in Collections:
Cell and Cryobiology Research Group

Full metadata record

DC FieldValue Language
dc.contributor.authorKarunakaran, R.en_GB
dc.contributor.authorMauchline, T.H.en_GB
dc.contributor.authorHosie, Arthur H.F.en_GB
dc.contributor.authorPoole, Philip S.en_GB
dc.date.accessioned2013-06-14T10:23:26Z-
dc.date.available2013-06-14T10:23:26Z-
dc.date.issued2005-
dc.identifier.citationKarunakaran, R., Mauchline, T. H., Hosie, A.H.F. and Poole, P.S. (2005) 'A family of promoter probe vectors incorporating autofluorescent and chromogenic reporter proteins for studying gene expression in gram-negative bacteria', Microbiology, 151 (10),pp.3249-3256en_GB
dc.identifier.issn1350-0872-
dc.identifier.issn1465-2080-
dc.identifier.doi10.1099/mic.0.28311-0-
dc.identifier.urihttp://hdl.handle.net/10547/293938-
dc.description.abstractA series of promoter probe vectors for use in Gram-negative bacteria has been made in two broad-host-range vectors, pOT (pBBR replicon) and pJP2 (incP replicon). Reporter fusions can be made to gfpUV, gfpmut3.1, unstable gfpmut3.1 variants (LAA, LVA, AAV and ASV), gfp+, dsRed2, dsRedT.3, dsRedT.4, mRFP1, gusA or lacZ. The two vector families, pOT and pJP2, are compatible with one another and share the same polylinker for facile interchange of promoter regions. Vectors based on pJP2 have the advantage of being ultra-stable in the environment due to the presence of the parABCDE genes. As a confirmation of their usefulness, the dicarboxylic acid transport system promoter (dctAp) was cloned into a pOT (pRU1097)- and a pJP2 (pRU1156)-based vector and shown to be expressed by Rhizobium leguminosarum in infection threads of vetch. This indicates the presence of dicarboxylates at the earliest stages of nodule formation.en_GB
dc.language.isoenen
dc.publisherSociety for General Microbiologyen_GB
dc.relation.urlhttp://mic.sgmjournals.org/cgi/doi/10.1099/mic.0.28311-0en_GB
dc.rightsArchived with thanks to Microbiologyen_GB
dc.subjectautofluorescent proteinen_GB
dc.subjectfluorescence-activated cell sorteren_GB
dc.subjectgreen fluorescent proteinen_GB
dc.titleA family of promoter probe vectors incorporating autofluorescent and chromogenic reporter proteins for studying gene expression in gram-negative bacteriaen
dc.typeArticleen
dc.identifier.journalMicrobiologyen_GB
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