2.50
Hdl Handle:
http://hdl.handle.net/10547/293936
Title:
Expression of P450 enzymes in rat whole skin and cultured epidermal keratinocytes
Authors:
Zhu, Zheying; Hotchkiss, Sharon A.; Boobis, Alan R.; Edwards, Robert J.
Abstract:
The complement and level of expression of P450 enzymes in male Fischer F344 rat whole skin and cultured keratinocytes were investigated using a panel of mono-specific antibodies. In whole skin microsomal fraction, immunoreactive bands corresponding to CYP2B12, CYP2C13, CYP2D1, CYP2D4, CYP2E1, CYP3A1, and CYP3A2 were detected whereas CYP1A1, CYP1A2, and CYP2C12 were absent. Skin levels were all between 0.1% and 4.7% of those found in liver, except for CYP2D4, which was not detected in liver. Keratinocytes were isolated from rat skin, cultured for up to 42 days, and changes in the levels of CYP3A1, CYP3A2, and CYP2E1 determined. Levels were low in isolated keratinocytes, but they increased markedly in culture, reaching a maximum at 10–14 days, where they were similar to those found in fresh skin. This suggests that primary keratinocytes grown in culture for 10–14 days may provide a useful experimental model to study P450-catalysed metabolism of xenobiotics in skin.
Citation:
Zhu, Z., Hotchkiss, S.A., Boobis, A.R. and Edwards, R.J. (2002) 'Expression of P450 enzymes in rat whole skin and cultured epidermal keratinocytes', Biochemical and Biophysical Research Communications, 297(1),pp.65-70
Publisher:
Elsevier
Journal:
Biochemical and Biophysical Research Communications
Issue Date:
2002
URI:
http://hdl.handle.net/10547/293936
DOI:
10.1016/S0006-291X(02)02134-4
Additional Links:
http://www.ncbi.nlm.nih.gov/pubmed/12220509; http://www.sciencedirect.com/science/article/pii/S0006291X02021344
Type:
Article
Language:
en
ISSN:
0006-291X
Appears in Collections:
Cell and Cryobiology Research Group

Full metadata record

DC FieldValue Language
dc.contributor.authorZhu, Zheyingen_GB
dc.contributor.authorHotchkiss, Sharon A.en_GB
dc.contributor.authorBoobis, Alan R.en_GB
dc.contributor.authorEdwards, Robert J.en_GB
dc.date.accessioned2013-06-14T09:46:30Zen
dc.date.available2013-06-14T09:46:30Zen
dc.date.issued2002en
dc.identifier.citationZhu, Z., Hotchkiss, S.A., Boobis, A.R. and Edwards, R.J. (2002) 'Expression of P450 enzymes in rat whole skin and cultured epidermal keratinocytes', Biochemical and Biophysical Research Communications, 297(1),pp.65-70en_GB
dc.identifier.issn0006-291Xen
dc.identifier.doi10.1016/S0006-291X(02)02134-4en
dc.identifier.urihttp://hdl.handle.net/10547/293936en
dc.description.abstractThe complement and level of expression of P450 enzymes in male Fischer F344 rat whole skin and cultured keratinocytes were investigated using a panel of mono-specific antibodies. In whole skin microsomal fraction, immunoreactive bands corresponding to CYP2B12, CYP2C13, CYP2D1, CYP2D4, CYP2E1, CYP3A1, and CYP3A2 were detected whereas CYP1A1, CYP1A2, and CYP2C12 were absent. Skin levels were all between 0.1% and 4.7% of those found in liver, except for CYP2D4, which was not detected in liver. Keratinocytes were isolated from rat skin, cultured for up to 42 days, and changes in the levels of CYP3A1, CYP3A2, and CYP2E1 determined. Levels were low in isolated keratinocytes, but they increased markedly in culture, reaching a maximum at 10–14 days, where they were similar to those found in fresh skin. This suggests that primary keratinocytes grown in culture for 10–14 days may provide a useful experimental model to study P450-catalysed metabolism of xenobiotics in skin.en_GB
dc.language.isoenen
dc.publisherElsevieren_GB
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pubmed/12220509en_GB
dc.relation.urlhttp://www.sciencedirect.com/science/article/pii/S0006291X02021344en
dc.subjectcytochrome P450en_GB
dc.subjectrat skinen_GB
dc.subjectcell cultureen_GB
dc.subjectkeratinocytesen_GB
dc.subjectCYPen_GB
dc.titleExpression of P450 enzymes in rat whole skin and cultured epidermal keratinocytesen
dc.typeArticleen
dc.identifier.journalBiochemical and Biophysical Research Communicationsen_GB
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