Distance measurements in the borderline region of applicability of CW EPR and DEER: a model study on a homologous series of spin-labelled peptides.

2.50
Hdl Handle:
http://hdl.handle.net/10547/228939
Title:
Distance measurements in the borderline region of applicability of CW EPR and DEER: a model study on a homologous series of spin-labelled peptides.
Authors:
Banham, Janet E.; Baker, Christopher M.; Ceola, Stefano; Day, Iain J.; Grant, Guy H.; Groenen, Edgar J.J.; Rodgers, Christopher T.; Jeschke, Gunnar; Timmel, Christiane R.
Abstract:
Inter-spin distances between 1 nm and 4.5 nm are measured by continuous wave (CW) and pulsed electron paramagnetic resonance (EPR) methods for a series of nitroxide-spin-labelled peptides. The upper distance limit for measuring dipolar coupling by the broadening of the CW spectrum and the lower distance limit for the present optimally-adjusted double electron electron resonance (DEER) set-up are determined and found to be both around 1.6-1.9 nm. The methods for determining distances and corresponding distributions from CW spectral line broadening are reviewed and further developed. Also, the work shows that a correction factor is required for the analysis of inter-spin distances below approximately 2 nm for DEER measurements and this is calculated using the density matrix formalism.
Affiliation:
Centre for Advanced Electron Spin Resonance, University of Oxford, OX1 3QR, UK.
Citation:
Distance measurements in the borderline region of applicability of CW EPR and DEER: a model study on a homologous series of spin-labelled peptides. 2008, 191 (2):202-218 J. Magn. Reson.
Publisher:
Elsevier
Journal:
Journal of magnetic resonance (San Diego, Calif. : 1997)
Issue Date:
Apr-2008
URI:
http://hdl.handle.net/10547/228939
DOI:
10.1016/j.jmr.2007.11.023
PubMed ID:
18280189
Additional Links:
http://www.ncbi.nlm.nih.gov/pubmed/18280189
Type:
Article
Language:
en
ISSN:
1090-7807
Appears in Collections:
Cell and Cryobiology Research Group

Full metadata record

DC FieldValue Language
dc.contributor.authorBanham, Janet E.en_GB
dc.contributor.authorBaker, Christopher M.en_GB
dc.contributor.authorCeola, Stefanoen_GB
dc.contributor.authorDay, Iain J.en_GB
dc.contributor.authorGrant, Guy H.en_GB
dc.contributor.authorGroenen, Edgar J.J.en_GB
dc.contributor.authorRodgers, Christopher T.en_GB
dc.contributor.authorJeschke, Gunnaren_GB
dc.contributor.authorTimmel, Christiane R.en_GB
dc.date.accessioned2012-06-14T11:51:19Z-
dc.date.available2012-06-14T11:51:19Z-
dc.date.issued2008-04-
dc.identifier.citationDistance measurements in the borderline region of applicability of CW EPR and DEER: a model study on a homologous series of spin-labelled peptides. 2008, 191 (2):202-218 J. Magn. Reson.en_GB
dc.identifier.issn1090-7807-
dc.identifier.pmid18280189-
dc.identifier.doi10.1016/j.jmr.2007.11.023-
dc.identifier.urihttp://hdl.handle.net/10547/228939-
dc.description.abstractInter-spin distances between 1 nm and 4.5 nm are measured by continuous wave (CW) and pulsed electron paramagnetic resonance (EPR) methods for a series of nitroxide-spin-labelled peptides. The upper distance limit for measuring dipolar coupling by the broadening of the CW spectrum and the lower distance limit for the present optimally-adjusted double electron electron resonance (DEER) set-up are determined and found to be both around 1.6-1.9 nm. The methods for determining distances and corresponding distributions from CW spectral line broadening are reviewed and further developed. Also, the work shows that a correction factor is required for the analysis of inter-spin distances below approximately 2 nm for DEER measurements and this is calculated using the density matrix formalism.en_GB
dc.language.isoenen
dc.publisherElsevieren_GB
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pubmed/18280189en_GB
dc.rightsArchived with thanks to Journal of magnetic resonance (San Diego, Calif. : 1997)en_GB
dc.subjectDEERen_GB
dc.subjectEPR spectroscopyen_GB
dc.subjectstructure elucidationen_GB
dc.subjectdipolar interactionsen_GB
dc.subjectspin labelsen_GB
dc.subject.meshAlgorithms-
dc.subject.meshComputer Simulation-
dc.subject.meshElectron Spin Resonance Spectroscopy-
dc.subject.meshModels, Chemical-
dc.subject.meshPeptides-
dc.subject.meshSpin Labels-
dc.titleDistance measurements in the borderline region of applicability of CW EPR and DEER: a model study on a homologous series of spin-labelled peptides.en
dc.typeArticleen
dc.contributor.departmentCentre for Advanced Electron Spin Resonance, University of Oxford, OX1 3QR, UK.en_GB
dc.identifier.journalJournal of magnetic resonance (San Diego, Calif. : 1997)en_GB

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