Proteomic analysis of human breast cell lines using SELDI-TOF MS shows that mixtures of estrogenic compounds exhibit simple similar action (concentration additivity)

2.50
Hdl Handle:
http://hdl.handle.net/10547/228934
Title:
Proteomic analysis of human breast cell lines using SELDI-TOF MS shows that mixtures of estrogenic compounds exhibit simple similar action (concentration additivity)
Authors:
Zhu, Zheying; Edwards, Robert J.; Boobis, Alan R.
Abstract:
Endocrine modulating chemicals in the environment are possible causative agents of a number of human diseases. Many of these compounds act on the same molecular target, and hence risk assessment requires consideration of their possible combined effects. Here, we studied the combined effects of 17beta-estradiol, genistein, bisphenol A and endosulfan on MCF-7 cells. Full concentration-effect curves for cell proliferation were obtained for each compound and used to identify appropriate concentrations for investigating the effects of binary mixtures of the compounds. Protein profiling by surface-enhanced laser desorption/ionization time of flight mass spectrometry was performed to identify responsive proteins. Treatment with each of the compounds produced similar protein profile changes. Prominent and consistent changes were seen in 12 protein ions. Cell proliferation and protein levels responded monotonically to the estrogens, with identifiable no observable effect concentrations in all cases. Binary mixtures of the compounds produced effects on cell proliferation and on each of the responsive protein ions that were fully consistent with concentration additivity. Thus, no reason to deviate from the application of the principles of dose-response and dose additivity in the risk assessment of combinations of estrogenic compounds was found in this study.
Affiliation:
Imperial College London
Citation:
Zhu, Z., Edwards, R.J., Boobis, A.R. (2008) 'Proteomic analysis of human breast cell lines using SELDI-TOF MS shows that mixtures of estrogenic compounds exhibit simple similar action (concentration additivity)' Toxicol. Lett. 181 (2):93-103
Publisher:
Elsevier
Journal:
Toxicology letters
Issue Date:
26-Sep-2008
URI:
http://hdl.handle.net/10547/228934
DOI:
10.1016/j.toxlet.2008.07.006
PubMed ID:
18675332
Additional Links:
http://www.ncbi.nlm.nih.gov/pubmed/18675332
Type:
Article
Language:
en
ISSN:
0378-4274
Appears in Collections:
Cell and Cryobiology Research Group

Full metadata record

DC FieldValue Language
dc.contributor.authorZhu, Zheyingen_GB
dc.contributor.authorEdwards, Robert J.en_GB
dc.contributor.authorBoobis, Alan R.en_GB
dc.date.accessioned2012-06-14T10:47:39Zen
dc.date.available2012-06-14T10:47:39Zen
dc.date.issued2008-09-26en
dc.identifier.citationZhu, Z., Edwards, R.J., Boobis, A.R. (2008) 'Proteomic analysis of human breast cell lines using SELDI-TOF MS shows that mixtures of estrogenic compounds exhibit simple similar action (concentration additivity)' Toxicol. Lett. 181 (2):93-103en_GB
dc.identifier.issn0378-4274en
dc.identifier.pmid18675332en
dc.identifier.doi10.1016/j.toxlet.2008.07.006en
dc.identifier.urihttp://hdl.handle.net/10547/228934en
dc.description.abstractEndocrine modulating chemicals in the environment are possible causative agents of a number of human diseases. Many of these compounds act on the same molecular target, and hence risk assessment requires consideration of their possible combined effects. Here, we studied the combined effects of 17beta-estradiol, genistein, bisphenol A and endosulfan on MCF-7 cells. Full concentration-effect curves for cell proliferation were obtained for each compound and used to identify appropriate concentrations for investigating the effects of binary mixtures of the compounds. Protein profiling by surface-enhanced laser desorption/ionization time of flight mass spectrometry was performed to identify responsive proteins. Treatment with each of the compounds produced similar protein profile changes. Prominent and consistent changes were seen in 12 protein ions. Cell proliferation and protein levels responded monotonically to the estrogens, with identifiable no observable effect concentrations in all cases. Binary mixtures of the compounds produced effects on cell proliferation and on each of the responsive protein ions that were fully consistent with concentration additivity. Thus, no reason to deviate from the application of the principles of dose-response and dose additivity in the risk assessment of combinations of estrogenic compounds was found in this study.en_GB
dc.language.isoenen
dc.publisherElsevieren_GB
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pubmed/18675332en_GB
dc.subject.meshBreast Neoplasmsen
dc.subject.meshCell Line, Tumoren
dc.subject.meshCell Proliferationen
dc.subject.meshDose-Response Relationship, Drugen
dc.subject.meshEstrogensen
dc.subject.meshFemaleen
dc.subject.meshHumansen
dc.subject.meshNeoplasm Proteinsen
dc.subject.meshProteomicsen
dc.subject.meshSpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionizationen
dc.titleProteomic analysis of human breast cell lines using SELDI-TOF MS shows that mixtures of estrogenic compounds exhibit simple similar action (concentration additivity)en
dc.typeArticleen
dc.contributor.departmentImperial College Londonen_GB
dc.identifier.journalToxicology lettersen_GB

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