Identification of estrogen-responsive proteins in MCF-7 human breast cancer cells using label-free quantitative proteomics

2.50
Hdl Handle:
http://hdl.handle.net/10547/228916
Title:
Identification of estrogen-responsive proteins in MCF-7 human breast cancer cells using label-free quantitative proteomics
Authors:
Zhu, Zheying; Boobis, Alan R.; Edwards, Robert J.
Abstract:
17beta-Estradiol (E(2)) is a key regulatory steroid hormone that is involved in the control of a number of developmental and other functions. The aim of the present work was to identify estrogen-dependent proteomic changes by determining the levels of expressed proteins in MCF-7 human breast cancer cells following treatment with E(2). A number of methods exist for differential analysis of complex proteomic mixtures. Here, a label-free mass spectrometric approach comparing the ion intensities of tryptic peptides was adopted, which was combined with prefractionation of whole cell lysate proteins by 1-D SDS-PAGE. Using this approach, 60 proteins were found to be affected by E(2). These comprised 55 up-regulated and five down-regulated proteins. These proteins varied widely in their physiochemical properties with pIs of 4-12 and molecular weights of 9-500 kDa. Pathway analysis revealed that the majority of changes were related and together describe an up-regulated pathway consistent with the events of cell proliferation. The quantitative approach used here is relatively straightforward, avoids the use of costly labelling reagents, was reproducible within acceptable limits and has a linear response over a useful concentration range.
Affiliation:
Department of Experimental Medicine and Toxicology, Division of Investigative Science, Imperial College London, Hammersmith Campus, London, UK.
Citation:
Zhu, Z. et al (2008) 'Identification of estrogen-responsive proteins in MCF-7 human breast cancer cells using label-free quantitative proteomics' Proteomics 8 (10):1987-2005
Publisher:
Wiley-VCH Verlag
Journal:
Proteomics
Issue Date:
May-2008
URI:
http://hdl.handle.net/10547/228916
DOI:
10.1002/pmic.200700901
PubMed ID:
18491314
Additional Links:
http://www.ncbi.nlm.nih.gov/pubmed/18491314
Type:
Article
Language:
en
ISSN:
1615-9861
Appears in Collections:
Cell and Cryobiology Research Group

Full metadata record

DC FieldValue Language
dc.contributor.authorZhu, Zheyingen_GB
dc.contributor.authorBoobis, Alan R.en_GB
dc.contributor.authorEdwards, Robert J.en_GB
dc.date.accessioned2012-06-14T10:50:47Zen
dc.date.available2012-06-14T10:50:47Zen
dc.date.issued2008-05en
dc.identifier.citationZhu, Z. et al (2008) 'Identification of estrogen-responsive proteins in MCF-7 human breast cancer cells using label-free quantitative proteomics' Proteomics 8 (10):1987-2005en_GB
dc.identifier.issn1615-9861en
dc.identifier.pmid18491314en
dc.identifier.doi10.1002/pmic.200700901en
dc.identifier.urihttp://hdl.handle.net/10547/228916en
dc.description.abstract17beta-Estradiol (E(2)) is a key regulatory steroid hormone that is involved in the control of a number of developmental and other functions. The aim of the present work was to identify estrogen-dependent proteomic changes by determining the levels of expressed proteins in MCF-7 human breast cancer cells following treatment with E(2). A number of methods exist for differential analysis of complex proteomic mixtures. Here, a label-free mass spectrometric approach comparing the ion intensities of tryptic peptides was adopted, which was combined with prefractionation of whole cell lysate proteins by 1-D SDS-PAGE. Using this approach, 60 proteins were found to be affected by E(2). These comprised 55 up-regulated and five down-regulated proteins. These proteins varied widely in their physiochemical properties with pIs of 4-12 and molecular weights of 9-500 kDa. Pathway analysis revealed that the majority of changes were related and together describe an up-regulated pathway consistent with the events of cell proliferation. The quantitative approach used here is relatively straightforward, avoids the use of costly labelling reagents, was reproducible within acceptable limits and has a linear response over a useful concentration range.en_GB
dc.language.isoenen
dc.publisherWiley-VCH Verlagen_GB
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pubmed/18491314en_GB
dc.subject.meshCell Line, Tumoren
dc.subject.meshDown-Regulationen
dc.subject.meshElectrophoresis, Polyacrylamide Gelen
dc.subject.meshEstradiolen
dc.subject.meshEstrogensen
dc.subject.meshHumansen
dc.subject.meshMass Spectrometryen
dc.subject.meshProteinsen
dc.subject.meshProteomicsen
dc.subject.meshUp-Regulationen
dc.titleIdentification of estrogen-responsive proteins in MCF-7 human breast cancer cells using label-free quantitative proteomicsen
dc.typeArticleen
dc.contributor.departmentDepartment of Experimental Medicine and Toxicology, Division of Investigative Science, Imperial College London, Hammersmith Campus, London, UK.en_GB
dc.identifier.journalProteomicsen_GB

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