Characterization of a novel sialic acid transporter of the sodium solute symporter (SSS) family and in vivo comparison with known bacterial sialic acid transporters.

2.50
Hdl Handle:
http://hdl.handle.net/10547/228911
Title:
Characterization of a novel sialic acid transporter of the sodium solute symporter (SSS) family and in vivo comparison with known bacterial sialic acid transporters.
Authors:
Severi, Emmanuele; Hosie, Arthur H.F. ( 0000-0002-1327-7901 ) ; Hawkhead, Judith A.; Thomas, Gavin H.
Abstract:
The function of sialic acids in the biology of bacterial pathogens is reflected by the diverse range of solute transporters that can recognize these sugar acids. Here, we use an Escherichia coliDeltananT strain to characterize the function of known and proposed bacterial sialic acid transporters. We discover that the STM1128 gene from Salmonella enterica serovar Typhimurium, which encodes a member of the sodium solute symporter family, is able to restore growth on sialic acid to the DeltananT strain and is able to transport [(14)C]-sialic acid. Using the DeltananT genetic background, we performed a direct in vivo comparison of the transport properties of the STM1128 protein with those of sialic acid transporters of the major facilitator superfamily and tripartite ATP-independent periplasmic families, E. coli NanT and Haemophilus influenzae SiaPQM, respectively. This revealed that both STM1128 and SiaPQM are sodium-dependent and, unlike SiaPQM, both STM1128 and NanT are reversible secondary carriers, demonstrating qualitative functional differences in the properties of sialic acid transporters used by bacteria that colonize humans.
Affiliation:
University of York
Citation:
Characterization of a novel sialic acid transporter of the sodium solute symporter (SSS) family and in vivo comparison with known bacterial sialic acid transporters. 2010, 304 (1):47-54 FEMS Microbiol. Lett.
Publisher:
Blackwell Publishing
Journal:
FEMS microbiology letters
Issue Date:
Mar-2010
URI:
http://hdl.handle.net/10547/228911
DOI:
10.1111/j.1574-6968.2009.01881.x
PubMed ID:
20100283
Additional Links:
http://www.ncbi.nlm.nih.gov/pubmed/20100283
Type:
Article
Language:
en
ISSN:
1574-6968
Appears in Collections:
Cell and Cryobiology Research Group

Full metadata record

DC FieldValue Language
dc.contributor.authorSeveri, Emmanueleen_GB
dc.contributor.authorHosie, Arthur H.F.en_GB
dc.contributor.authorHawkhead, Judith A.en_GB
dc.contributor.authorThomas, Gavin H.en_GB
dc.date.accessioned2012-06-14T10:06:06Z-
dc.date.available2012-06-14T10:06:06Z-
dc.date.issued2010-03-
dc.identifier.citationCharacterization of a novel sialic acid transporter of the sodium solute symporter (SSS) family and in vivo comparison with known bacterial sialic acid transporters. 2010, 304 (1):47-54 FEMS Microbiol. Lett.en_GB
dc.identifier.issn1574-6968-
dc.identifier.pmid20100283-
dc.identifier.doi10.1111/j.1574-6968.2009.01881.x-
dc.identifier.urihttp://hdl.handle.net/10547/228911-
dc.description.abstractThe function of sialic acids in the biology of bacterial pathogens is reflected by the diverse range of solute transporters that can recognize these sugar acids. Here, we use an Escherichia coliDeltananT strain to characterize the function of known and proposed bacterial sialic acid transporters. We discover that the STM1128 gene from Salmonella enterica serovar Typhimurium, which encodes a member of the sodium solute symporter family, is able to restore growth on sialic acid to the DeltananT strain and is able to transport [(14)C]-sialic acid. Using the DeltananT genetic background, we performed a direct in vivo comparison of the transport properties of the STM1128 protein with those of sialic acid transporters of the major facilitator superfamily and tripartite ATP-independent periplasmic families, E. coli NanT and Haemophilus influenzae SiaPQM, respectively. This revealed that both STM1128 and SiaPQM are sodium-dependent and, unlike SiaPQM, both STM1128 and NanT are reversible secondary carriers, demonstrating qualitative functional differences in the properties of sialic acid transporters used by bacteria that colonize humans.en_GB
dc.language.isoenen
dc.publisherBlackwell Publishingen_GB
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pubmed/20100283en_GB
dc.rightsArchived with thanks to FEMS microbiology lettersen_GB
dc.subject.meshBacterial Proteins-
dc.subject.meshEscherichia coli-
dc.subject.meshEscherichia coli Proteins-
dc.subject.meshHaemophilus influenzae-
dc.subject.meshHumans-
dc.subject.meshMembrane Transport Proteins-
dc.subject.meshN-Acetylneuraminic Acid-
dc.subject.meshOrganic Anion Transporters-
dc.subject.meshSalmonella typhimurium-
dc.subject.meshSymporters-
dc.titleCharacterization of a novel sialic acid transporter of the sodium solute symporter (SSS) family and in vivo comparison with known bacterial sialic acid transporters.en
dc.typeArticleen
dc.contributor.departmentUniversity of Yorken_GB
dc.identifier.journalFEMS microbiology lettersen_GB

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