5.00
Hdl Handle:
http://hdl.handle.net/10547/228752
Title:
Effect of chilling on sox2, sox3 and sox19a gene expression in zebrafish (Danio rerio) embryos
Authors:
Desai, Kunjan ( 0000-0002-4204-5931 ) ; Spikings, Emma ( 0000-0001-8387-2098 ) ; Zhang, Tiantian
Abstract:
Zebrafish embryos have not been cryopreserved due to their structural limitations. Although embryo survival rates have been used as the measured outcome for most of the cryopreservation protocols studied, there are very limited data available at the molecular level. This study focused on the effect of chilling and subsequent warming on gene expression of sox2, sox3 and sox19a which play vital roles in the development of zebrafish embryos. A quantitative RT-PCR approach was used to investigate gene expression following chilling at 0°C for up to 180 min. The effect on gene expression was also studied during a 180 min warming period after chilling for 30 or 60 min. There were significant decreases in sox2 (up to 4-fold) and sox3 (up to 3-fold) expressions following chilling. Significant increases in gene expressions of sox2 (up to 2-fold), sox3 (up to 33-fold) and sox19a (up to 25-fold) were observed during warming in the embryos that had been chilled for 30 min. Similarly, significant increases were observed in sox2 (up to 3-fold) and sox3 (up to 2-fold) during warming in embryos that had been chilled for 60 min. These increases may be explained by compensation for the suppression observed during chilling and/or to activate repair mechanisms or maintain homeostasis.
Affiliation:
University of Bedfordshire
Citation:
Desai, K., Spikings, E., Zhang, T. (2011) 'Effect of chilling on sox2, sox3 and sox19a gene expression in zebrafish (Danio rerio) embryos' Cryobiology 63 (2):96-103
Publisher:
Elsevier
Journal:
Cryobiology
Issue Date:
Oct-2011
URI:
http://hdl.handle.net/10547/228752
DOI:
10.1016/j.cryobiol.2011.07.001
PubMed ID:
21820425
Additional Links:
http://www.ncbi.nlm.nih.gov/pubmed/21820425; http://www.sciencedirect.com/science/article/pii/S0011224011001167
Type:
Article
Language:
en
ISSN:
1090-2392
Appears in Collections:
Environmental Monitoring Research Group

Full metadata record

DC FieldValue Language
dc.contributor.authorDesai, Kunjanen_GB
dc.contributor.authorSpikings, Emmaen_GB
dc.contributor.authorZhang, Tiantianen_GB
dc.date.accessioned2012-06-13T11:02:35Zen
dc.date.available2012-06-13T11:02:35Zen
dc.date.issued2011-10en
dc.identifier.citationDesai, K., Spikings, E., Zhang, T. (2011) 'Effect of chilling on sox2, sox3 and sox19a gene expression in zebrafish (Danio rerio) embryos' Cryobiology 63 (2):96-103en_GB
dc.identifier.issn1090-2392en
dc.identifier.pmid21820425en
dc.identifier.doi10.1016/j.cryobiol.2011.07.001en
dc.identifier.urihttp://hdl.handle.net/10547/228752en
dc.description.abstractZebrafish embryos have not been cryopreserved due to their structural limitations. Although embryo survival rates have been used as the measured outcome for most of the cryopreservation protocols studied, there are very limited data available at the molecular level. This study focused on the effect of chilling and subsequent warming on gene expression of sox2, sox3 and sox19a which play vital roles in the development of zebrafish embryos. A quantitative RT-PCR approach was used to investigate gene expression following chilling at 0°C for up to 180 min. The effect on gene expression was also studied during a 180 min warming period after chilling for 30 or 60 min. There were significant decreases in sox2 (up to 4-fold) and sox3 (up to 3-fold) expressions following chilling. Significant increases in gene expressions of sox2 (up to 2-fold), sox3 (up to 33-fold) and sox19a (up to 25-fold) were observed during warming in the embryos that had been chilled for 30 min. Similarly, significant increases were observed in sox2 (up to 3-fold) and sox3 (up to 2-fold) during warming in embryos that had been chilled for 60 min. These increases may be explained by compensation for the suppression observed during chilling and/or to activate repair mechanisms or maintain homeostasis.en_GB
dc.language.isoenen
dc.publisherElsevieren_GB
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pubmed/21820425en_GB
dc.relation.urlhttp://www.sciencedirect.com/science/article/pii/S0011224011001167en
dc.subjectzebrafishen_GB
dc.subjectcryobiologyen
dc.subjectembryosen_GB
dc.subjectchillingen_GB
dc.subjectgene expressionen_GB
dc.subjectsox genesen_GB
dc.subjectwarmingen_GB
dc.subjectDanio rerioen
dc.subject.meshAnimalsen
dc.subject.meshCold Temperatureen
dc.subject.meshCryopreservationen
dc.subject.meshEmbryo, Nonmammalianen
dc.subject.meshGene Expressionen
dc.subject.meshSOX Transcription Factorsen
dc.subject.meshSOXB1 Transcription Factorsen
dc.subject.meshZebrafishen
dc.subject.meshZebrafish Proteinsen
dc.titleEffect of chilling on sox2, sox3 and sox19a gene expression in zebrafish (Danio rerio) embryosen
dc.typeArticleen
dc.contributor.departmentUniversity of Bedfordshireen_GB
dc.identifier.journalCryobiologyen_GB
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