Study on the mitochondrial activity and membrane potential after exposing later stage oocytes of two gorgonian corals (Junceella juncea and Junceella fragilis) to cryoprotectants.

2.50
Hdl Handle:
http://hdl.handle.net/10547/228730
Title:
Study on the mitochondrial activity and membrane potential after exposing later stage oocytes of two gorgonian corals (Junceella juncea and Junceella fragilis) to cryoprotectants.
Authors:
Tsai, Sujune; Spikings, Emma ( 0000-0001-8387-2098 ) ; Huang, I-C.; Lin, C.
Abstract:
Coral reefs provide a valuable habitat for many economically valuable fish and invertebrates. However, they are in serious jeopardy, threatened by increasing over-exploitation, pollution, habitat destruction, disease and global climate change. Here, we examined the effect of cryoprotectant exposure on mitochondrial activity and membrane potential in coral oocytes in order to find suitable cryoprotectants towards their successful cryopreservation. According to the No Observed Effect Concentrations (NOECs), methanol was found to be the least toxic cryoprotectant whilst DMSO was the most toxic cryoprotectant. The results also demonstrated that there were no significant differences (p > 0.05) in ATP concentrations between Junceella juncea and Junceella fragilis after exposure to all concentrations of all cryoprotectants for 30 min. Using confocal microscopy, JC-1 (5,50,6,60-tetrachloro-1,10,3,30-tetraethyl-imidacarbocyanine iodide) staining indicated that the mitochondrial membrane potential of Junceella fragilis oocytes reduced after 1 M and 2 M methanol treatment and a loss of the mitochondrial distribution pattern and poor green fluorescence after 3M methanol treatment. Therefore, even oocytes that show no adverse effect of cryoprotectants on survival might suffer some more subtle impacts. The results obtained from this study will provide a basis for development of protocols to cryopreserve the oocytes of gorgonian corals.
Affiliation:
Mingdao University
Citation:
Tsai, S., Spikings, E., Huang, I-C., Lin, C. (2011) 'Study on the mitochondrial activity and membrane potential after exposing later stage oocytes of two gorgonian corals (Junceella juncea and Junceella fragilis) to cryoprotectants' Cryo Letters 32 (1):1-12
Publisher:
Cryo Letters
Journal:
Cryo letters
Issue Date:
Jan-2011
URI:
http://hdl.handle.net/10547/228730
PubMed ID:
21468448
Additional Links:
http://www.ncbi.nlm.nih.gov/pubmed/21468448; http://www.ingentaconnect.com/content/cryo/cryo/2011/00000032/00000001/art00001?token=00541bf705f864ec38341333c4a2f7a6c6a533847462148763b734f6d6222346b6268763050214d7767c
Type:
Article
Language:
en
ISSN:
0143-2044
Appears in Collections:
Cell and Cryobiology Research Group

Full metadata record

DC FieldValue Language
dc.contributor.authorTsai, Sujuneen_GB
dc.contributor.authorSpikings, Emmaen_GB
dc.contributor.authorHuang, I-C.en_GB
dc.contributor.authorLin, C.en_GB
dc.date.accessioned2012-06-13T10:52:29Zen
dc.date.available2012-06-13T10:52:29Zen
dc.date.issued2011-01en
dc.identifier.citationTsai, S., Spikings, E., Huang, I-C., Lin, C. (2011) 'Study on the mitochondrial activity and membrane potential after exposing later stage oocytes of two gorgonian corals (Junceella juncea and Junceella fragilis) to cryoprotectants' Cryo Letters 32 (1):1-12en_GB
dc.identifier.issn0143-2044en
dc.identifier.pmid21468448en
dc.identifier.urihttp://hdl.handle.net/10547/228730en
dc.description.abstractCoral reefs provide a valuable habitat for many economically valuable fish and invertebrates. However, they are in serious jeopardy, threatened by increasing over-exploitation, pollution, habitat destruction, disease and global climate change. Here, we examined the effect of cryoprotectant exposure on mitochondrial activity and membrane potential in coral oocytes in order to find suitable cryoprotectants towards their successful cryopreservation. According to the No Observed Effect Concentrations (NOECs), methanol was found to be the least toxic cryoprotectant whilst DMSO was the most toxic cryoprotectant. The results also demonstrated that there were no significant differences (p > 0.05) in ATP concentrations between Junceella juncea and Junceella fragilis after exposure to all concentrations of all cryoprotectants for 30 min. Using confocal microscopy, JC-1 (5,50,6,60-tetrachloro-1,10,3,30-tetraethyl-imidacarbocyanine iodide) staining indicated that the mitochondrial membrane potential of Junceella fragilis oocytes reduced after 1 M and 2 M methanol treatment and a loss of the mitochondrial distribution pattern and poor green fluorescence after 3M methanol treatment. Therefore, even oocytes that show no adverse effect of cryoprotectants on survival might suffer some more subtle impacts. The results obtained from this study will provide a basis for development of protocols to cryopreserve the oocytes of gorgonian corals.en_GB
dc.language.isoenen
dc.publisherCryo Lettersen_GB
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pubmed/21468448en_GB
dc.relation.urlhttp://www.ingentaconnect.com/content/cryo/cryo/2011/00000032/00000001/art00001?token=00541bf705f864ec38341333c4a2f7a6c6a533847462148763b734f6d6222346b6268763050214d7767cen
dc.subjectgorgonian coralen_GB
dc.subjectoocyteen_GB
dc.subjectmitochondrial membrane potentialen_GB
dc.subjectcryoprotectantsen_GB
dc.subject.meshAdenosine Triphosphateen
dc.subject.meshAnimalsen
dc.subject.meshAnthozoaen
dc.subject.meshCoral Reefsen
dc.subject.meshCryoprotective Agentsen
dc.subject.meshDimethyl Sulfoxideen
dc.subject.meshFemaleen
dc.subject.meshFluorescenceen
dc.subject.meshMembrane Potential, Mitochondrialen
dc.subject.meshMethanolen
dc.subject.meshMicroscopy, Confocalen
dc.subject.meshMitochondriaen
dc.subject.meshOocyte Retrievalen
dc.subject.meshOocytesen
dc.subject.meshSpecies Specificityen
dc.titleStudy on the mitochondrial activity and membrane potential after exposing later stage oocytes of two gorgonian corals (Junceella juncea and Junceella fragilis) to cryoprotectants.en
dc.typeArticleen
dc.contributor.departmentMingdao Universityen_GB
dc.identifier.journalCryo lettersen_GB
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