Effects of the slow cooling during cryopreservation on the survival and morphology of Taiwan shoveljaw carp (Varicorhinus barbatulus) spermatozoa

2.50
Hdl Handle:
http://hdl.handle.net/10547/228726
Title:
Effects of the slow cooling during cryopreservation on the survival and morphology of Taiwan shoveljaw carp (Varicorhinus barbatulus) spermatozoa
Authors:
Tsai, Sujune; Spikings, Emma ( 0000-0001-8387-2098 ) ; Hwang, Ching-Chiang; Lin, Chiashin
Abstract:
Over the past decades, pollution, overfishing, and habitat degradation have driven the population size of Taiwan shoveljaw carp down markedly in Taiwan. Cryopreservation is a useful tool which could be used to maintain genetic resources to protect and preserve this endemic species. Four cryoprotectants [dimethyl sulphoxide (DMSO), dimethylacetamide (DMA), glycerol and methanol] and six freezing rates (0.5, 1, 2, 4, 8, 16 °C min-1) were tested in order to develop an optimal controlled slow-freezing protocol for Taiwan shoveljaw carp spermatozoa. Samples were subsequently examined under the scanning electron microscope to reveal whether cryopreservation had affected their ultrastructural morphology. The highest survival rate (50.1 ± 2.0%) was observed with a freezing rate of 8 °C min-1 in 1M DMSO, using SYBR-14 + PI staining. Fertility and hatching rate results using frozen-thawed spermatozoa (90.2 ± 2.2% and 22.3 ± 2.5%, respectively) were not significantly different from results with fresh spermatozoa. After cryopreservation, 21.0 ± 1.6% of frozen-thawed spermatozoa had mid-piece swelling and rupture of the head. Cryopreservation might, therefore, slightly affect Taiwan shoveljaw carp spermatozoa in terms of morphological change. However, these alterations could be compensated by using large enough numbers of normally functioning frozen-thawed spermatozoa to achieve a standard equal to fresh spermatozoa. This is the first report of successful cryopreservation of Taiwan shoveljaw carp spermatozoa using a controlled slow-cooling method.
Affiliation:
Mingdao University; University of Bedfordshire; National Museum of Marine Biology & Aquarium, Taiwan
Citation:
Tsai, S., Spikings, E., Hwang, C-C., Lin, C. (2010) 'Effects of the slow cooling during cryopreservation on the survival and morphology of Taiwan shoveljaw carp (Varicorhinus barbatulus ) spermatozoa' Aquatic Living Resources 23 (1):119-124
Publisher:
EDP Sciences
Journal:
Aquatic Living Resources
Issue Date:
Jan-2010
URI:
http://hdl.handle.net/10547/228726
DOI:
10.1051/alr/2009055
Additional Links:
http://www.alr-journal.org/10.1051/alr/2009055
Type:
Article
Language:
en
ISSN:
0990-7440; 1765-2952
Appears in Collections:
Cell and Cryobiology Research Group

Full metadata record

DC FieldValue Language
dc.contributor.authorTsai, Sujuneen_GB
dc.contributor.authorSpikings, Emmaen_GB
dc.contributor.authorHwang, Ching-Chiangen_GB
dc.contributor.authorLin, Chiashinen_GB
dc.date.accessioned2012-06-13T10:41:33Zen
dc.date.available2012-06-13T10:41:33Zen
dc.date.issued2010-01en
dc.identifier.citationTsai, S., Spikings, E., Hwang, C-C., Lin, C. (2010) 'Effects of the slow cooling during cryopreservation on the survival and morphology of Taiwan shoveljaw carp (Varicorhinus barbatulus ) spermatozoa' Aquatic Living Resources 23 (1):119-124en_GB
dc.identifier.issn0990-7440en
dc.identifier.issn1765-2952en
dc.identifier.doi10.1051/alr/2009055en
dc.identifier.urihttp://hdl.handle.net/10547/228726en
dc.description.abstractOver the past decades, pollution, overfishing, and habitat degradation have driven the population size of Taiwan shoveljaw carp down markedly in Taiwan. Cryopreservation is a useful tool which could be used to maintain genetic resources to protect and preserve this endemic species. Four cryoprotectants [dimethyl sulphoxide (DMSO), dimethylacetamide (DMA), glycerol and methanol] and six freezing rates (0.5, 1, 2, 4, 8, 16 °C min-1) were tested in order to develop an optimal controlled slow-freezing protocol for Taiwan shoveljaw carp spermatozoa. Samples were subsequently examined under the scanning electron microscope to reveal whether cryopreservation had affected their ultrastructural morphology. The highest survival rate (50.1 ± 2.0%) was observed with a freezing rate of 8 °C min-1 in 1M DMSO, using SYBR-14 + PI staining. Fertility and hatching rate results using frozen-thawed spermatozoa (90.2 ± 2.2% and 22.3 ± 2.5%, respectively) were not significantly different from results with fresh spermatozoa. After cryopreservation, 21.0 ± 1.6% of frozen-thawed spermatozoa had mid-piece swelling and rupture of the head. Cryopreservation might, therefore, slightly affect Taiwan shoveljaw carp spermatozoa in terms of morphological change. However, these alterations could be compensated by using large enough numbers of normally functioning frozen-thawed spermatozoa to achieve a standard equal to fresh spermatozoa. This is the first report of successful cryopreservation of Taiwan shoveljaw carp spermatozoa using a controlled slow-cooling method.en_GB
dc.language.isoenen
dc.publisherEDP Sciencesen_GB
dc.relation.urlhttp://www.alr-journal.org/10.1051/alr/2009055en_GB
dc.rightsArchived with thanks to Aquatic Living Resourcesen_GB
dc.subjectcryopreservationen_GB
dc.subjectscanning electron microscopeen
dc.subjectfreezing rateen
dc.subjectfertilisationen
dc.subjectTaiwan shoveljaw carpen
dc.subjectCyprinidaeen
dc.titleEffects of the slow cooling during cryopreservation on the survival and morphology of Taiwan shoveljaw carp (Varicorhinus barbatulus) spermatozoaen
dc.typeArticleen
dc.contributor.departmentMingdao Universityen_GB
dc.contributor.departmentUniversity of Bedfordshireen
dc.contributor.departmentNational Museum of Marine Biology & Aquarium, Taiwanen
dc.identifier.journalAquatic Living Resourcesen_GB
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