Effect of methanol and Me2SO exposure on mitochondrial activity and distribution in stage III ovarian follicles of zebrafish (Danio rerio).

2.50
Hdl Handle:
http://hdl.handle.net/10547/228725
Title:
Effect of methanol and Me2SO exposure on mitochondrial activity and distribution in stage III ovarian follicles of zebrafish (Danio rerio).
Authors:
Zampolla, Tiziana; Spikings, Emma ( 0000-0001-8387-2098 ) ; Zhang, Tiantian; Rawson, David M.
Abstract:
In this study the effect of cryoprotectants that have been shown to be the least toxic to zebrafish ovarian follicles (methanol and Me(2)SO), on mitochondria of stage III ovarian follicles was evaluated. The mitochondrial distributional arrangement, mitochondrial membrane potential, mtDNA copy number, ATP levels and ADP/ATP ratios were assessed following exposure to cryoprotectants for 30 min at room temperature. Results obtained by confocal microscopy showed that 30 min exposure to 2M methanol induced a loss of membrane potential, although viability tests showed no decrease in survival even after 5h post-exposure incubation. Higher concentrations of methanol (3 and 4M) induced not only a decrease in mitochondrial membrane potential but also the loss of mitochondrial distributional arrangement, which suggested a compromised mitochondrial function. Furthermore 3 and 4M treatments resulted in a decrease in viability assessed by Fluorescein diacetate-Propidium iodide (FDA-PI) and in a decrease in mtDNA copy number and ADP/ATP ratio after 5h incubation following methanol exposure, indicating a delayed effect. The use of Me(2)SO, which is considered to be a more toxic CPA to zebrafish ovarian follicles than methanol, caused a decrease in viability and a sustained decrease in ATP levels accompanied by failure to maintain mtDNA copy number within 1h post-exposure incubation. These results indicated that even CPAs that are considered to have no toxicity as determined by Trypan blue (TB) and FDA-PI tests can have a deleterious effect on mitochondrial activity, potentially compromising oocyte growth and embryo development.
Affiliation:
LIRANS Institute of Research in the Applied Natural Sciences, University of Bedfordshire, Luton, Bedfordshire, United Kingdom.
Citation:
Zampolla, T., Spikings, E., Zhang, T., Rawson, D.M. (2009) 'Effect of methanol and Me2SO exposure on mitochondrial activity and distribution in stage III ovarian follicles of zebrafish (Danio rerio)' Cryobiology 59 (2):188-194
Publisher:
Elsevier
Journal:
Cryobiology
Issue Date:
Oct-2009
URI:
http://hdl.handle.net/10547/228725
DOI:
10.1016/j.cryobiol.2009.07.002
PubMed ID:
19595995
Additional Links:
http://www.sciencedirect.com/science/article/pii/S0011224009000960
Type:
Article
Language:
en
ISSN:
1090-2392
Appears in Collections:
Cell and Cryobiology Research Group

Full metadata record

DC FieldValue Language
dc.contributor.authorZampolla, Tizianaen_GB
dc.contributor.authorSpikings, Emmaen_GB
dc.contributor.authorZhang, Tiantianen_GB
dc.contributor.authorRawson, David M.en_GB
dc.date.accessioned2012-06-13T10:34:56Zen
dc.date.available2012-06-13T10:34:56Zen
dc.date.issued2009-10en
dc.identifier.citationZampolla, T., Spikings, E., Zhang, T., Rawson, D.M. (2009) 'Effect of methanol and Me2SO exposure on mitochondrial activity and distribution in stage III ovarian follicles of zebrafish (Danio rerio)' Cryobiology 59 (2):188-194en_GB
dc.identifier.issn1090-2392en
dc.identifier.pmid19595995en
dc.identifier.doi10.1016/j.cryobiol.2009.07.002en
dc.identifier.urihttp://hdl.handle.net/10547/228725en
dc.description.abstractIn this study the effect of cryoprotectants that have been shown to be the least toxic to zebrafish ovarian follicles (methanol and Me(2)SO), on mitochondria of stage III ovarian follicles was evaluated. The mitochondrial distributional arrangement, mitochondrial membrane potential, mtDNA copy number, ATP levels and ADP/ATP ratios were assessed following exposure to cryoprotectants for 30 min at room temperature. Results obtained by confocal microscopy showed that 30 min exposure to 2M methanol induced a loss of membrane potential, although viability tests showed no decrease in survival even after 5h post-exposure incubation. Higher concentrations of methanol (3 and 4M) induced not only a decrease in mitochondrial membrane potential but also the loss of mitochondrial distributional arrangement, which suggested a compromised mitochondrial function. Furthermore 3 and 4M treatments resulted in a decrease in viability assessed by Fluorescein diacetate-Propidium iodide (FDA-PI) and in a decrease in mtDNA copy number and ADP/ATP ratio after 5h incubation following methanol exposure, indicating a delayed effect. The use of Me(2)SO, which is considered to be a more toxic CPA to zebrafish ovarian follicles than methanol, caused a decrease in viability and a sustained decrease in ATP levels accompanied by failure to maintain mtDNA copy number within 1h post-exposure incubation. These results indicated that even CPAs that are considered to have no toxicity as determined by Trypan blue (TB) and FDA-PI tests can have a deleterious effect on mitochondrial activity, potentially compromising oocyte growth and embryo development.en_GB
dc.language.isoenen
dc.publisherElsevieren_GB
dc.relation.urlhttp://www.sciencedirect.com/science/article/pii/S0011224009000960en_GB
dc.subjectcryoprotectantsen_GB
dc.subjectmitochondrial activityen
dc.subjectovarian folliclesen
dc.subjectzebrafishen
dc.subjectcryobiologyen
dc.subject.meshAdenosine Diphosphateen
dc.subject.meshAdenosine Triphosphateen
dc.subject.meshAnimalsen
dc.subject.meshCell Survivalen
dc.subject.meshCryoprotective Agentsen
dc.subject.meshDNA, Mitochondrialen
dc.subject.meshDimethyl Sulfoxideen
dc.subject.meshEmbryo, Nonmammalianen
dc.subject.meshFemaleen
dc.subject.meshMembrane Potential, Mitochondrialen
dc.subject.meshMethanolen
dc.subject.meshMitochondriaen
dc.subject.meshOvarian Follicleen
dc.subject.meshZebrafishen
dc.titleEffect of methanol and Me2SO exposure on mitochondrial activity and distribution in stage III ovarian follicles of zebrafish (Danio rerio).en
dc.typeArticleen
dc.contributor.departmentLIRANS Institute of Research in the Applied Natural Sciences, University of Bedfordshire, Luton, Bedfordshire, United Kingdom.en_GB
dc.identifier.journalCryobiologyen_GB
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