Effect of chilling and cryopreservation on expression of Pax genes in zebrafish (Danio rerio) embryos and blastomeres

2.50
Hdl Handle:
http://hdl.handle.net/10547/228724
Title:
Effect of chilling and cryopreservation on expression of Pax genes in zebrafish (Danio rerio) embryos and blastomeres
Authors:
Lin, C.; Spikings, Emma ( 0000-0001-8387-2098 ) ; Zhang, Tiantian; Rawson, David M.
Abstract:
Cryopreservation is now common practice in the fields of aquaculture, conservation and biomedicine. However, there is a lack of information on the effect of chilling and cryopreservation at the molecular level. In the present study, we used real-time RT-PCR analysis to determine the effect of chilling and cryopreservation on expression of Pax2a, Pax2b, Pax5 and Pax8 which constitute one subgroup of the Pax gene family. As intact embryos of zebrafish have not yet been successfully cryopreserved, we have used two alternatives: chilling of intact embryos and cryopreservation of isolated blastomeres. Cryopreservation was found to affect the normal pattern of gene expression in zebrafish embryonic blastomeres. The trends, profile changes, in expression of Pax2a and Pax5 occurred to a lesser extent in frozen-thawed blastomeres than in fresh blastomeres whilst the opposite was true for Pax8. The trends in expression of Pax2b were delayed in frozen-thawed blastomeres compared to fresh blastomeres. Cryopreservation can therefore disrupt normal gene expression patterns in zebrafish embryonic blastomeres which could have a detrimental effect on embryo development.
Affiliation:
University of Bedfordshire
Citation:
Lin, C., Spikings, E., Zhang, T., Rawson, D.M. (2009) 'Effect of chilling and cryopreservation on expression of Pax genes in zebrafish (Danio rerio) embryos and blastomeres' Cryobiology 59 (1):42-47
Publisher:
Elsevier
Journal:
Cryobiology
Issue Date:
Aug-2009
URI:
http://hdl.handle.net/10547/228724
DOI:
10.1016/j.cryobiol.2009.04.007
PubMed ID:
19397904
Additional Links:
http://www.ncbi.nlm.nih.gov/pubmed/19397904; http://www.sciencedirect.com/science/article/pii/S0011224009000376
Type:
Article
Language:
en
ISSN:
1090-2392
Appears in Collections:
Cell and Cryobiology Research Group

Full metadata record

DC FieldValue Language
dc.contributor.authorLin, C.en_GB
dc.contributor.authorSpikings, Emmaen_GB
dc.contributor.authorZhang, Tiantianen_GB
dc.contributor.authorRawson, David M.en_GB
dc.date.accessioned2012-06-13T10:32:04Zen
dc.date.available2012-06-13T10:32:04Zen
dc.date.issued2009-08en
dc.identifier.citationLin, C., Spikings, E., Zhang, T., Rawson, D.M. (2009) 'Effect of chilling and cryopreservation on expression of Pax genes in zebrafish (Danio rerio) embryos and blastomeres' Cryobiology 59 (1):42-47en_GB
dc.identifier.issn1090-2392en
dc.identifier.pmid19397904en
dc.identifier.doi10.1016/j.cryobiol.2009.04.007en
dc.identifier.urihttp://hdl.handle.net/10547/228724en
dc.description.abstractCryopreservation is now common practice in the fields of aquaculture, conservation and biomedicine. However, there is a lack of information on the effect of chilling and cryopreservation at the molecular level. In the present study, we used real-time RT-PCR analysis to determine the effect of chilling and cryopreservation on expression of Pax2a, Pax2b, Pax5 and Pax8 which constitute one subgroup of the Pax gene family. As intact embryos of zebrafish have not yet been successfully cryopreserved, we have used two alternatives: chilling of intact embryos and cryopreservation of isolated blastomeres. Cryopreservation was found to affect the normal pattern of gene expression in zebrafish embryonic blastomeres. The trends, profile changes, in expression of Pax2a and Pax5 occurred to a lesser extent in frozen-thawed blastomeres than in fresh blastomeres whilst the opposite was true for Pax8. The trends in expression of Pax2b were delayed in frozen-thawed blastomeres compared to fresh blastomeres. Cryopreservation can therefore disrupt normal gene expression patterns in zebrafish embryonic blastomeres which could have a detrimental effect on embryo development.en_GB
dc.language.isoenen
dc.publisherElsevieren_GB
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pubmed/19397904en_GB
dc.relation.urlhttp://www.sciencedirect.com/science/article/pii/S0011224009000376en
dc.subjectzebrafishen_GB
dc.subjectcryobiologyen
dc.subjectembryosen_GB
dc.subjectblastomeresen_GB
dc.subjectgene expressionen_GB
dc.subjectpaxen_GB
dc.subjectchillingen_GB
dc.subjectcryopreservationen_GB
dc.subject.meshAnimalsen
dc.subject.meshBlastomeresen
dc.subject.meshCold Temperatureen
dc.subject.meshCryopreservationen
dc.subject.meshCryoprotective Agentsen
dc.subject.meshEmbryo Culture Techniquesen
dc.subject.meshEmbryo, Nonmammalianen
dc.subject.meshFemaleen
dc.subject.meshFreezingen
dc.subject.meshGene Expression Profilingen
dc.subject.meshGene Expression Regulation, Developmentalen
dc.subject.meshMaleen
dc.subject.meshPaired Box Transcription Factorsen
dc.subject.meshTemperatureen
dc.subject.meshZebrafishen
dc.titleEffect of chilling and cryopreservation on expression of Pax genes in zebrafish (Danio rerio) embryos and blastomeresen
dc.typeArticleen
dc.contributor.departmentUniversity of Bedfordshireen_GB
dc.identifier.journalCryobiologyen_GB

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