2.50
Hdl Handle:
http://hdl.handle.net/10547/228723
Title:
Housekeeping genes for cryopreservation studies on zebrafish embryos and blastomeres.
Authors:
Lin, C.; Spikings, Emma ( 0000-0001-8387-2098 ) ; Zhang, Tiantian; Rawson, David M.
Abstract:
Cryopreservation success is usually analysed in terms of cell survival, although there are other potential effects that do not necessarily result in cell death. These include DNA damage, which could result in altered gene expression. Real-time reverse transcriptase PCR allows quantitative analysis of gene expression but usually requires analysis of a 'housekeeping' gene as an internal reference. As the stability of housekeeping genes varies significantly among different groups of samples, it is recommended that those chosen are validated for each different type of sample group. This study aimed to validate housekeeping genes for use in cryopreservation studies of zebrafish embryos. Seven potential housekeeping genes were analysed across fresh and chilled intact embryos and across fresh and frozen isolated blastomeres using the GeNorm and NormFinder software packages. Results suggest that combined use of beta-actin and EF1alpha as housekeeping genes would be suitable for cryopreservation studies on zebrafish embryos and blastomeres.
Affiliation:
LIRANS Research Institute, University of Bedfordshire, 250 Butterfield, Great Marlings, Luton, Bedfordshire, LU2 8DL, UK.
Citation:
Lin, C., Spikings, E., Zhang, T., Rawson, D.M. (2009) 'Housekeeping genes for cryopreservation studies on zebrafish embryos and blastomeres' Theriogenology 71 (7):1147-1155
Publisher:
Elsevier
Journal:
Theriogenology
Issue Date:
15-Apr-2009
URI:
http://hdl.handle.net/10547/228723
DOI:
10.1016/j.theriogenology.2008.12.013
PubMed ID:
19201018
Additional Links:
http://www.ncbi.nlm.nih.gov/pubmed/19201018; http://www.sciencedirect.com/science/article/pii/S0093691X08008054
Type:
Article
Language:
en
ISSN:
0093-691X
Appears in Collections:
Cell and Cryobiology Research Group

Full metadata record

DC FieldValue Language
dc.contributor.authorLin, C.en_GB
dc.contributor.authorSpikings, Emmaen_GB
dc.contributor.authorZhang, Tiantianen_GB
dc.contributor.authorRawson, David M.en_GB
dc.date.accessioned2012-06-13T10:29:31Zen
dc.date.available2012-06-13T10:29:31Zen
dc.date.issued2009-04-15en
dc.identifier.citationLin, C., Spikings, E., Zhang, T., Rawson, D.M. (2009) 'Housekeeping genes for cryopreservation studies on zebrafish embryos and blastomeres' Theriogenology 71 (7):1147-1155en_GB
dc.identifier.issn0093-691Xen
dc.identifier.pmid19201018en
dc.identifier.doi10.1016/j.theriogenology.2008.12.013en
dc.identifier.urihttp://hdl.handle.net/10547/228723en
dc.description.abstractCryopreservation success is usually analysed in terms of cell survival, although there are other potential effects that do not necessarily result in cell death. These include DNA damage, which could result in altered gene expression. Real-time reverse transcriptase PCR allows quantitative analysis of gene expression but usually requires analysis of a 'housekeeping' gene as an internal reference. As the stability of housekeeping genes varies significantly among different groups of samples, it is recommended that those chosen are validated for each different type of sample group. This study aimed to validate housekeeping genes for use in cryopreservation studies of zebrafish embryos. Seven potential housekeeping genes were analysed across fresh and chilled intact embryos and across fresh and frozen isolated blastomeres using the GeNorm and NormFinder software packages. Results suggest that combined use of beta-actin and EF1alpha as housekeeping genes would be suitable for cryopreservation studies on zebrafish embryos and blastomeres.en_GB
dc.language.isoenen
dc.publisherElsevieren_GB
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pubmed/19201018en_GB
dc.relation.urlhttp://www.sciencedirect.com/science/article/pii/S0093691X08008054en
dc.subjectcryopreservationen_GB
dc.subjecthousekeeping geneen
dc.subjectembryosen
dc.subjectblastomeresen
dc.subjectzebrafishen
dc.subject.meshAnimalsen
dc.subject.meshBlastomeresen
dc.subject.meshCryopreservationen
dc.subject.meshEmbryo, Nonmammalianen
dc.subject.meshGene Expression Regulation, Developmentalen
dc.subject.meshGenetic Markersen
dc.subject.meshZebrafishen
dc.titleHousekeeping genes for cryopreservation studies on zebrafish embryos and blastomeres.en
dc.typeArticleen
dc.contributor.departmentLIRANS Research Institute, University of Bedfordshire, 250 Butterfield, Great Marlings, Luton, Bedfordshire, LU2 8DL, UK.en_GB
dc.identifier.journalTheriogenologyen_GB

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