2.50
Hdl Handle:
http://hdl.handle.net/10547/228719
Title:
Mitochondria directly influence fertilisation outcome in the pig
Authors:
El Shourbagy, Shahinaz H.; Spikings, Emma ( 0000-0001-8387-2098 ) ; Freitas, Mariana; St John, Justin C.
Abstract:
The mitochondrion is explicitly involved in cytoplasmic regulation and is the cell's major generator of ATP. Our aim was to determine whether mitochondria alone could influence fertilisation outcome. In vitro, oocyte competence can be assessed through the presence of glucose-6-phosphate dehydrogenase (G6PD) as indicated by the dye, brilliant cresyl blue (BCB). Using porcine in vitro fertilisation (IVF), we have assessed oocyte maturation, cytoplasmic volume, fertilisation outcome, mitochondrial number as determined by mtDNA copy number, and whether mitochondria are uniformly distributed between blastomeres of each embryo. After staining with BCB, we observed a significant difference in cytoplasmic volume between BCB positive (BCB+) and BCB negative (BCB-) oocytes. There was also a significant difference in mtDNA copy number between fertilised and unfertilised oocytes and unequal mitochondrial segregation between blastomeres during early cleavage stages. Furthermore, we have supplemented BCB- oocytes with mitochondria from maternal relatives and observed a significant difference in fertilisation outcomes following both IVF and intracytoplasmic sperm injection (ICSI) between supplemented, sham-injected and non-treated BCB- oocytes. We have therefore demonstrated a relationship between oocyte maturity, cytoplasmic volume, and fertilisation outcome and mitochondrial content. These data suggest that mitochondrial number is important for fertilisation outcome and embryonic development. Furthermore, a mitochondrial pre-fertilisation threshold may ensure that, as mitochondria are diluted out during post-fertilisation cleavage, there are sufficient copies of mtDNA per blastomere to allow transmission of mtDNA to each cell of the post-implantation embryo after the initiation of mtDNA replication during the early postimplantation stages.
Affiliation:
University of Birmingham
Citation:
El Shourbagy, S.H., Spikings, E.C., Freitas, M., St John, J.C. (2006) 'Mitochondria directly influence fertilisation outcome in the pig', Reproduction 131 (2):233-45
Publisher:
Society for Reproduction and Fertility
Journal:
Reproduction (Cambridge, England)
Issue Date:
Feb-2006
URI:
http://hdl.handle.net/10547/228719
DOI:
10.1530/rep.1.00551
PubMed ID:
16452717
Additional Links:
http://www.ncbi.nlm.nih.gov/pubmed/16452717; http://www.reproduction-online.org/content/131/2/233
Type:
Article
Language:
en
ISSN:
1470-1626
Appears in Collections:
Cell and Cryobiology Research Group

Full metadata record

DC FieldValue Language
dc.contributor.authorEl Shourbagy, Shahinaz H.en_GB
dc.contributor.authorSpikings, Emmaen_GB
dc.contributor.authorFreitas, Marianaen_GB
dc.contributor.authorSt John, Justin C.en_GB
dc.date.accessioned2012-06-13T10:13:07Zen
dc.date.available2012-06-13T10:13:07Zen
dc.date.issued2006-02en
dc.identifier.citationEl Shourbagy, S.H., Spikings, E.C., Freitas, M., St John, J.C. (2006) 'Mitochondria directly influence fertilisation outcome in the pig', Reproduction 131 (2):233-45en_GB
dc.identifier.issn1470-1626en
dc.identifier.pmid16452717en
dc.identifier.doi10.1530/rep.1.00551en
dc.identifier.urihttp://hdl.handle.net/10547/228719en
dc.description.abstractThe mitochondrion is explicitly involved in cytoplasmic regulation and is the cell's major generator of ATP. Our aim was to determine whether mitochondria alone could influence fertilisation outcome. In vitro, oocyte competence can be assessed through the presence of glucose-6-phosphate dehydrogenase (G6PD) as indicated by the dye, brilliant cresyl blue (BCB). Using porcine in vitro fertilisation (IVF), we have assessed oocyte maturation, cytoplasmic volume, fertilisation outcome, mitochondrial number as determined by mtDNA copy number, and whether mitochondria are uniformly distributed between blastomeres of each embryo. After staining with BCB, we observed a significant difference in cytoplasmic volume between BCB positive (BCB+) and BCB negative (BCB-) oocytes. There was also a significant difference in mtDNA copy number between fertilised and unfertilised oocytes and unequal mitochondrial segregation between blastomeres during early cleavage stages. Furthermore, we have supplemented BCB- oocytes with mitochondria from maternal relatives and observed a significant difference in fertilisation outcomes following both IVF and intracytoplasmic sperm injection (ICSI) between supplemented, sham-injected and non-treated BCB- oocytes. We have therefore demonstrated a relationship between oocyte maturity, cytoplasmic volume, and fertilisation outcome and mitochondrial content. These data suggest that mitochondrial number is important for fertilisation outcome and embryonic development. Furthermore, a mitochondrial pre-fertilisation threshold may ensure that, as mitochondria are diluted out during post-fertilisation cleavage, there are sufficient copies of mtDNA per blastomere to allow transmission of mtDNA to each cell of the post-implantation embryo after the initiation of mtDNA replication during the early postimplantation stages.en_GB
dc.language.isoenen
dc.publisherSociety for Reproduction and Fertilityen_GB
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pubmed/16452717en_GB
dc.relation.urlhttp://www.reproduction-online.org/content/131/2/233en_GB
dc.rightsArchived with thanks to Reproduction (Cambridge, England)en_GB
dc.subject.meshAnimalsen
dc.subject.meshBlastomeresen
dc.subject.meshCytoplasmen
dc.subject.meshDNA, Mitochondrialen
dc.subject.meshFemaleen
dc.subject.meshFertilization in Vitroen
dc.subject.meshGlucosephosphate Dehydrogenaseen
dc.subject.meshMaleen
dc.subject.meshMitochondriaen
dc.subject.meshOocytesen
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen
dc.subject.meshSperm Injections, Intracytoplasmicen
dc.subject.meshSperm-Ovum Interactionsen
dc.subject.meshStaining and Labelingen
dc.subject.meshSwineen
dc.titleMitochondria directly influence fertilisation outcome in the pigen
dc.typeArticleen
dc.contributor.departmentUniversity of Birminghamen_GB
dc.identifier.journalReproduction (Cambridge, England)en_GB

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