Cytoskeleton proteins F-actin and tubulin distribution and interaction with mitochondria in the granulosa cells surrounding stage III zebrafish (Danio rerio) oocytes

2.50
Hdl Handle:
http://hdl.handle.net/10547/228293
Title:
Cytoskeleton proteins F-actin and tubulin distribution and interaction with mitochondria in the granulosa cells surrounding stage III zebrafish (Danio rerio) oocytes
Authors:
Zampolla, Tiziana; Spikings, Emma ( 0000-0001-8387-2098 ) ; Rawson, David M.; Zhang, Tiantian
Abstract:
The distributional arrangement of mitochondria in the granulosa cells surrounding stage III zebrafish oocyte has been reported as a contiguous aggregation of mitochondria at the margin of the each granulosa cell. The aim of the present study was to further investigate the mitochondrial distribution in the granulosa cell layer in stage III ovarian follicles and the interaction between mitochondria and cytoskeleton elements actin and tubulin. To determine mitochondrial distribution/transport, immunocytochemistry analysis of tubulin and mitochondrial COX-I was carried out along with phalloidin staining of polymerised F-actin. The follicles were also exposed to a range of conditions that are known to affect mitochondria and the cytoskeleton proteins actin and tubulin. The mitochondrial inhibitor FCCP, the anti-mitotic drug nocodazole, and actin polymerisation inhibitor cytochalasin B were used. Levels of ATP, mtDNA copy number, and viability assessed by Trypan blue were also studied after exposure to inhibitors in order to determine the relationship between mitochondrial distribution/activity and ATP production. F-actin showed a hexagonal-polygonal distribution surrounding the mitochondria in granulosa cells, with the F-actin network adjacent to the plasma membrane of each granulosa cell. Tubulin structure presented a less organised distribution than F-actin, it was sparse in the cytosol. Interaction between mitochondria and tubulin was found indicating that mitochondria and tubulin are colocalised in zebrafish ovarian follicles. The exposure of ovarian follicles to inhibitors induced the loss of mitochondrial structural integrity showing that mitochondria distribution in granulosa cells of stage III zebrafish ovarian follicles is determined by the microtubules network.
Affiliation:
University of Bedfordshire
Citation:
Zampolla, T., Spikings, E., Rawson, D.M., Zhang, T. (2011) 'Cytoskeleton proteins F-actin and tubulin distribution and interaction with mitochondria in the granulosa cells surrounding stage III zebrafish (Danio rerio) oocytes' Theriogenology 76 (6):1110-1119
Publisher:
Elsevier
Journal:
Theriogenology
Issue Date:
1-Oct-2011
URI:
http://hdl.handle.net/10547/228293
DOI:
10.1016/j.theriogenology.2011.05.018
PubMed ID:
21752457
Additional Links:
http://www.ncbi.nlm.nih.gov/pubmed/21752457; http://www.sciencedirect.com/science/article/pii/S0093691X11002524
Type:
Article
Language:
en
ISSN:
1879-3231
Appears in Collections:
Environmental Monitoring Research Group

Full metadata record

DC FieldValue Language
dc.contributor.authorZampolla, Tizianaen_GB
dc.contributor.authorSpikings, Emmaen_GB
dc.contributor.authorRawson, David M.en_GB
dc.contributor.authorZhang, Tiantianen_GB
dc.date.accessioned2012-06-11T08:25:43Zen
dc.date.available2012-06-11T08:25:43Zen
dc.date.issued2011-10-01en
dc.identifier.citationZampolla, T., Spikings, E., Rawson, D.M., Zhang, T. (2011) 'Cytoskeleton proteins F-actin and tubulin distribution and interaction with mitochondria in the granulosa cells surrounding stage III zebrafish (Danio rerio) oocytes' Theriogenology 76 (6):1110-1119en_GB
dc.identifier.issn1879-3231en
dc.identifier.pmid21752457en
dc.identifier.doi10.1016/j.theriogenology.2011.05.018en
dc.identifier.urihttp://hdl.handle.net/10547/228293en
dc.description.abstractThe distributional arrangement of mitochondria in the granulosa cells surrounding stage III zebrafish oocyte has been reported as a contiguous aggregation of mitochondria at the margin of the each granulosa cell. The aim of the present study was to further investigate the mitochondrial distribution in the granulosa cell layer in stage III ovarian follicles and the interaction between mitochondria and cytoskeleton elements actin and tubulin. To determine mitochondrial distribution/transport, immunocytochemistry analysis of tubulin and mitochondrial COX-I was carried out along with phalloidin staining of polymerised F-actin. The follicles were also exposed to a range of conditions that are known to affect mitochondria and the cytoskeleton proteins actin and tubulin. The mitochondrial inhibitor FCCP, the anti-mitotic drug nocodazole, and actin polymerisation inhibitor cytochalasin B were used. Levels of ATP, mtDNA copy number, and viability assessed by Trypan blue were also studied after exposure to inhibitors in order to determine the relationship between mitochondrial distribution/activity and ATP production. F-actin showed a hexagonal-polygonal distribution surrounding the mitochondria in granulosa cells, with the F-actin network adjacent to the plasma membrane of each granulosa cell. Tubulin structure presented a less organised distribution than F-actin, it was sparse in the cytosol. Interaction between mitochondria and tubulin was found indicating that mitochondria and tubulin are colocalised in zebrafish ovarian follicles. The exposure of ovarian follicles to inhibitors induced the loss of mitochondrial structural integrity showing that mitochondria distribution in granulosa cells of stage III zebrafish ovarian follicles is determined by the microtubules network.en_GB
dc.language.isoenen
dc.publisherElsevieren_GB
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pubmed/21752457en_GB
dc.relation.urlhttp://www.sciencedirect.com/science/article/pii/S0093691X11002524en
dc.subjectcytoskeletonproteinsen_GB
dc.subjectzebrafishen_GB
dc.subjectovarian follicleen_GB
dc.subjectmitochondriaen_GB
dc.subject.meshActinsen
dc.subject.meshAdenosine Triphosphateen
dc.subject.meshAnimalsen
dc.subject.meshCarbonyl Cyanide p-Trifluoromethoxyphenylhydrazoneen
dc.subject.meshCytochalasin Ben
dc.subject.meshDNA, Mitochondrialen
dc.subject.meshFemaleen
dc.subject.meshGene Dosageen
dc.subject.meshGranulosa Cellsen
dc.subject.meshMitochondriaen
dc.subject.meshNocodazoleen
dc.subject.meshOocytesen
dc.subject.meshOvarian Follicleen
dc.subject.meshTubulinen
dc.subject.meshZebrafishen
dc.titleCytoskeleton proteins F-actin and tubulin distribution and interaction with mitochondria in the granulosa cells surrounding stage III zebrafish (Danio rerio) oocytesen
dc.typeArticleen
dc.contributor.departmentUniversity of Bedfordshireen_GB
dc.identifier.journalTheriogenologyen_GB

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