2.50
Hdl Handle:
http://hdl.handle.net/10547/225951
Title:
Daily hypoxia increases basal monocyte HSP72 expression in healthy human subjects.
Authors:
Taylor, Lee; Midgley, Adrian W.; Chrismas, Bryna; Hilman, Angela R.; Madden, Leigh A.; Vince, Rebecca V.; McNaughton, Lars R.
Abstract:
Heat shock protein 72 (HSP72) performs vital roles within the body at rest and during periods of stress. In vitro, research demonstrates HSP72 induction in response to hypoxia. Recently, in vivo, an acute hypoxic exposure (75 min at 2,980 m) was sufficient to induce significant increases in monocyte expressed HSP72 (mHSP72) and a marker of oxidative stress in healthy human subjects. The purpose of the current study was to identify the impact of 10 consecutive days of hypoxic exposures (75 min at 2,980 m) on mHSP72 and erythropoietin (EPO) expression, markers of oxidative stress, and maximal oxygen consumption in graded incremental aerobic exercise. Eight male subjects were exposed to daily normobaric hypoxic exposures for 75 min at 2,980 m for 10 consecutive days, commencing and ceasing at 0930 and 1045, respectively. This stressor was sufficient to induce significant increases in mHSP72, which was significantly elevated from day 2 of the hypoxic exposures until 48 h post-final exposure. Notably, this increase had an initial rapid (30% day on day compared to baseline) and final slow phase (16% day on day compared to baseline) of expression. The authors postulate that 7-day hypoxic exposure in this manner would be sufficient to induce near maximum hypoxia-mediated basal mHSP72 expression. Elevated levels of mHSP72 are associated with acquired thermotolerance and provide cross tolerance to non-related stressors in vivo, the protocol used here may provide a useful tool for elevating mHSP72 in vivo. Aside from these major findings, significant transient daily elevations were seen in a marker of oxidative stress, alongside sustained increases in EPO expression. However, no physiologically significant changes were seen in maximal oxygen consumption or time to exhaustion.
Affiliation:
University of Bedfordshire
Citation:
Taylor L., Midgley A.W., Chrismas B., Hilman A.R., Madden L.A., Vince R.V., and McNaughton L.R. (2011) 'Daily hypoxia increases basal monocyte HSP72 expression in healthy human subjects', Amino Acids, 40(2) pp.393-401.
Journal:
Amino Acids
Issue Date:
Feb-2011
URI:
http://hdl.handle.net/10547/225951
DOI:
10.1007/s00726-010-0644-x
PubMed ID:
20552383
Additional Links:
http://link.springer.com/article/10.1007/s00726-010-0644-x
Type:
Article
Language:
en
ISSN:
1438-2199
Appears in Collections:
Applied Sport and Exercise Physiology

Full metadata record

DC FieldValue Language
dc.contributor.authorTaylor, Leeen_GB
dc.contributor.authorMidgley, Adrian W.en_GB
dc.contributor.authorChrismas, Brynaen_GB
dc.contributor.authorHilman, Angela R.en_GB
dc.contributor.authorMadden, Leigh A.en_GB
dc.contributor.authorVince, Rebecca V.en_GB
dc.contributor.authorMcNaughton, Lars R.en_GB
dc.date.accessioned2012-05-25T08:52:40Zen
dc.date.available2012-05-25T08:52:40Zen
dc.date.issued2011-02en
dc.identifier.citationTaylor L., Midgley A.W., Chrismas B., Hilman A.R., Madden L.A., Vince R.V., and McNaughton L.R. (2011) 'Daily hypoxia increases basal monocyte HSP72 expression in healthy human subjects', Amino Acids, 40(2) pp.393-401.en_GB
dc.identifier.issn1438-2199en
dc.identifier.pmid20552383en
dc.identifier.doi10.1007/s00726-010-0644-xen
dc.identifier.urihttp://hdl.handle.net/10547/225951en
dc.description.abstractHeat shock protein 72 (HSP72) performs vital roles within the body at rest and during periods of stress. In vitro, research demonstrates HSP72 induction in response to hypoxia. Recently, in vivo, an acute hypoxic exposure (75 min at 2,980 m) was sufficient to induce significant increases in monocyte expressed HSP72 (mHSP72) and a marker of oxidative stress in healthy human subjects. The purpose of the current study was to identify the impact of 10 consecutive days of hypoxic exposures (75 min at 2,980 m) on mHSP72 and erythropoietin (EPO) expression, markers of oxidative stress, and maximal oxygen consumption in graded incremental aerobic exercise. Eight male subjects were exposed to daily normobaric hypoxic exposures for 75 min at 2,980 m for 10 consecutive days, commencing and ceasing at 0930 and 1045, respectively. This stressor was sufficient to induce significant increases in mHSP72, which was significantly elevated from day 2 of the hypoxic exposures until 48 h post-final exposure. Notably, this increase had an initial rapid (30% day on day compared to baseline) and final slow phase (16% day on day compared to baseline) of expression. The authors postulate that 7-day hypoxic exposure in this manner would be sufficient to induce near maximum hypoxia-mediated basal mHSP72 expression. Elevated levels of mHSP72 are associated with acquired thermotolerance and provide cross tolerance to non-related stressors in vivo, the protocol used here may provide a useful tool for elevating mHSP72 in vivo. Aside from these major findings, significant transient daily elevations were seen in a marker of oxidative stress, alongside sustained increases in EPO expression. However, no physiologically significant changes were seen in maximal oxygen consumption or time to exhaustion.en_GB
dc.language.isoenen
dc.relation.urlhttp://link.springer.com/article/10.1007/s00726-010-0644-xen
dc.rightsArchived with thanks to Amino acidsen_GB
dc.subject.meshAdolescenten
dc.subject.meshAnoxiaen
dc.subject.meshCells, Cultureden
dc.subject.meshErythropoietinen
dc.subject.meshHSP72 Heat-Shock Proteinsen
dc.subject.meshHumansen
dc.subject.meshMaleen
dc.subject.meshMonocytesen
dc.subject.meshOxidative Stressen
dc.subject.meshOxygen Consumptionen
dc.subject.meshUp-Regulationen
dc.subject.meshYoung Adulten
dc.titleDaily hypoxia increases basal monocyte HSP72 expression in healthy human subjects.en
dc.typeArticleen
dc.contributor.departmentUniversity of Bedfordshireen_GB
dc.identifier.journalAmino Acidsen_GB

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